Literature DB >> 21356882

Phenotypic analysis of Arabidopsis mutants: trypan blue stain for fungi, oomycetes, and dead plant cells.

Saskia van Wees.   

Abstract

INTRODUCTIONTrypan blue stains vasculature, dead plant cells, and fungal and oomycete hyphae. It is useful for assessing the extent of colonization of tissue, and for detecting microlesions present in certain lesion-mimic mutants. Trypan blue staining requires chloral hydrate for destaining, which is inconvenient, because it is a controlled substance. The chloral hydrate can be replaced with 1:2 lactophenol:ethanol, but the background staining will be higher than it is when chloral hydrate is used.

Entities:  

Year:  2008        PMID: 21356882     DOI: 10.1101/pdb.prot4982

Source DB:  PubMed          Journal:  CSH Protoc        ISSN: 1559-6095


  25 in total

1.  GLUTAMATE RECEPTOR-LIKE genes mediate leaf-to-leaf wound signalling.

Authors:  Seyed A R Mousavi; Adeline Chauvin; François Pascaud; Stephan Kellenberger; Edward E Farmer
Journal:  Nature       Date:  2013-08-22       Impact factor: 49.962

2.  Natural variation in timing of stress-responsive gene expression predicts heterosis in intraspecific hybrids of Arabidopsis.

Authors:  Marisa Miller; Qingxin Song; Xiaoli Shi; Thomas E Juenger; Z Jeffrey Chen
Journal:  Nat Commun       Date:  2015-07-08       Impact factor: 14.919

3.  Functional Analysis of Plant Defense Suppression and Activation by the Xanthomonas Core Type III Effector XopX.

Authors:  William Stork; Jung-Gun Kim; Mary Beth Mudgett
Journal:  Mol Plant Microbe Interact       Date:  2015-02       Impact factor: 4.171

4.  Loss of CRWN Nuclear Proteins Induces Cell Death and Salicylic Acid Defense Signaling.

Authors:  Junsik Choi; Susan R Strickler; Eric J Richards
Journal:  Plant Physiol       Date:  2019-01-29       Impact factor: 8.340

5.  Direct and Indirect Visualization of Bacterial Effector Delivery into Diverse Plant Cell Types during Infection.

Authors:  Elizabeth Henry; Tania Y Toruño; Alain Jauneau; Laurent Deslandes; Gitta Coaker
Journal:  Plant Cell       Date:  2017-06-09       Impact factor: 11.277

6.  The Arabidopsis Mitochondrial Protease FtSH4 Is Involved in Leaf Senescence via Regulation of WRKY-Dependent Salicylic Acid Accumulation and Signaling.

Authors:  Shengchun Zhang; Cui Li; Rui Wang; Yaxue Chen; Si Shu; Ruihua Huang; Daowei Zhang; Jian Li; Shi Xiao; Nan Yao; Chengwei Yang
Journal:  Plant Physiol       Date:  2017-03-01       Impact factor: 8.340

7.  XopR T3SS-effector of Xanthomonas oryzae pv. oryzae suppresses cell death-mediated plant defense response during bacterial blight development in rice.

Authors:  Geeta Verma; Kalyan K Mondal; Aditya Kulshreshtha; Manju Sharma
Journal:  3 Biotech       Date:  2019-06-18       Impact factor: 2.406

8.  The actin-related Protein2/3 complex regulates mitochondrial-associated calcium signaling during salt stress in Arabidopsis.

Authors:  Yi Zhao; Zhen Pan; Yan Zhang; Xiaolu Qu; Yuguo Zhang; Yongqing Yang; Xiangning Jiang; Shanjin Huang; Ming Yuan; Karen S Schumaker; Yan Guo
Journal:  Plant Cell       Date:  2013-11-26       Impact factor: 11.277

9.  Time-Course Transcriptome Analysis of Arabidopsis Siliques Discloses Genes Essential for Fruit Development and Maturation.

Authors:  Chiara Mizzotti; Lisa Rotasperti; Marco Moretto; Luca Tadini; Francesca Resentini; Bianca M Galliani; Massimo Galbiati; Kristof Engelen; Paolo Pesaresi; Simona Masiero
Journal:  Plant Physiol       Date:  2018-10-01       Impact factor: 8.340

10.  Heterologous expression of wheat TaRUB1 gene enhances disease resistance in Arabidopsis thaliana.

Authors:  Yanlin Yang; Wenqiang Wang; Tian Xu; Na Liu; Honggang Wang; Deshun Feng
Journal:  Plant Cell Rep       Date:  2017-10-14       Impact factor: 4.570

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