Literature DB >> 21356827

Mapping protein distributions on polytene chromosomes by immunostaining.

Renato Paro.   

Abstract

INTRODUCTIONThe formidable size and structure of polytene chromosomes allow mapping of chromosomal protein distributions at very high resolution. This protocol describes the preparation of polytene chromosomes from Drosophila larvae, immunostaining of the chromosomes with a protein of interest, and counterstaining with Giemsa and Hoechst.

Entities:  

Year:  2008        PMID： 21356827     DOI： 10.1101/pdb.prot4714

Source DB:  PubMed          Journal:  CSH Protoc        ISSN： 1559-6095


  4 in total

1.  Chromosomal binding sites of the homeotic cofactor Homothorax.

Authors:  Lior Cohen; Adi Salzberg
Journal:  Mol Genet Genomics       Date:  2008-05-15       Impact factor: 3.291

2.  Preparation of Drosophila Polytene Chromosomes, Followed by Immunofluorescence Analysis of Chromatin Structure by Multi-fluorescence Correlations.

Authors:  Terra M Kuhn; Shawn C Little; Maya Capelson
Journal:  Bio Protoc       Date:  2020-07-05

3.  Pho dynamically interacts with Spt5 to facilitate transcriptional switches at the hsp70 locus.

Authors:  Allwyn Pereira; Renato Paro
Journal:  Epigenetics Chromatin       Date:  2017-12-06       Impact factor: 4.954

4.  The Drosophila MLR COMPASS complex is essential for programming cis-regulatory information and maintaining epigenetic memory during development.

Authors:  Claudia B Zraly; Abdul Zakkar; John Hertenstein Perez; Jeffrey Ng; Kevin P White; Matthew Slattery; Andrew K Dingwall
Journal:  Nucleic Acids Res       Date:  2020-04-17       Impact factor: 16.971
  4 in total

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