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Literature DB >> 21356742

Whole-mount in situ hybridization in amphimedon.

Claire Larroux¹, Bryony Fahey, Maja Adamska, Gemma S Richards, Marie Gauthier, Kathryn Green, Erica Lovas, Bernard M Degnan.

Abstract

INTRODUCTIONDevelopmental gene expression is analyzed predominantly via whole-mount in situ hybridization using digoxigenin-labeled RNA probes. This protocol describes how to perform this procedure in Amphimedon queenslandica, including fixation, hybridization, and sectioning of embryonic, larval, and post-larval juvenile stages.

Entities: Chemical Species

Year: 2008 PMID： 21356742 DOI： 10.1101/pdb.prot5096

Source DB: PubMed Journal: CSH Protoc ISSN： 1559-6095

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Cited

8 in total

1. Blue-light-receptive cryptochrome is expressed in a sponge eye lacking neurons and opsin.

Authors: Ajna S Rivera; Nuri Ozturk; Bryony Fahey; David C Plachetzki; Bernard M Degnan; Aziz Sancar; Todd H Oakley
Journal: J Exp Biol Date: 2012-04-15 Impact factor: 3.312

2. Staining and Tracking Methods for Studying Sponge Cell Dynamics.

Authors: Carole Borchiellini; Sandie M Degnan; Emilie Le Goff; Caroline Rocher; Amélie Vernale; Stephen Baghdiguian; Nina Séjourné; Florent Marschal; André Le Bivic; Nelly Godefroy; Bernard M Degnan; Emmanuelle Renard
Journal: Methods Mol Biol Date: 2021

3. The expression of Delta ligands in the sponge Amphimedon queenslandica suggests an ancient role for Notch signaling in metazoan development.

Authors: Gemma S Richards; Bernard M Degnan
Journal: Evodevo Date: 2012-07-23 Impact factor: 2.250

4. Genome-wide analysis of the sox family in the calcareous sponge Sycon ciliatum: multiple genes with unique expression patterns.

Authors: Sofia Fortunato; Marcin Adamski; Brith Bergum; Corina Guder; Signe Jordal; Sven Leininger; Christin Zwafink; Hans Tore Rapp; Maja Adamska
Journal: Evodevo Date: 2012-07-23 Impact factor: 2.250

Whole-mount in situ hybridization in amphimedon.

1. Blue-light-receptive cryptochrome is expressed in a sponge eye lacking neurons and opsin.

2. Staining and Tracking Methods for Studying Sponge Cell Dynamics.

3. The expression of Delta ligands in the sponge Amphimedon queenslandica suggests an ancient role for Notch signaling in metazoan development.

4. Genome-wide analysis of the sox family in the calcareous sponge Sycon ciliatum: multiple genes with unique expression patterns.

5. An ancient role for nitric oxide in regulating the animal pelagobenthic life cycle: evidence from a marine sponge.

6. Sponge Long Non-Coding RNAs Are Expressed in Specific Cell Types and Conserved Networks.

7. Independent evolution of striated muscles in cnidarians and bilaterians.

8. The ontogeny of choanocyte chambers during metamorphosis in the demosponge Amphimedon queenslandica.