Apoptin gene transfer via modified wheat histone H4 facilitates apoptosis of human ovarian cancer cells.

Nonviral approaches have been used extensively for intracellular gene transfer and gene therapy. A modified wheat histone H4 protein, H4TL (H4-TAT-LHRH), as a protein-based gene delivery vector that was able to form stable complexes with plasmid DNA and increase gene delivery efficiency has been described previously. In this study, H4TL has been used to deliver apoptin gene into a human ovarian carcinoma cell line HO8910. After transfection, increased expression of apoptin at both mRNA and protein levels was detected in HO8910 cells, accompanied by reduced rate of growth of HO8910 cells in vitro and the loss of mitochondrial membrane potential in these cells. These data demonstrate that H4TL-mediated transfer of apoptin initiates mitochondrial death pathway in ovarian cancer cells and suggest a novel therapeutic strategy for cancer.