| Literature DB >> 21354049 |
Koen D Quint1, Daan T Geraets, Henk A M van den Munckhof, Maurits N C de Koning, Vitaly Smelov, Willem J G Melchers, Henry J C de Vries, Servaas A Morré, Chris J M Meijer, Dirk C J G van Alewijk, Leen-Jan van Doorn, Wim G V Quint.
Abstract
A novel Chlamydia trachomatis (Ct) microsphere suspension (MS) assay was evaluated for identification of the different serovars, using the same PCR primer set established for the Ct Detection and genoTyping assay. Both assays can detect and identify all 14 major serovars (A, B/Ba, C, D/Da, E, F, G/Ga, H, I/Ia, J, K, L1, L2/L2a, and L3) and one genovariant of serovar J. The probe specificity for the Ct-MS assay was determined using 14 Ct reference strains and 1 clinical isolate from a genovariant of serovar J. Also, the Ct-MS assay and the Ct detection and genoTyping assay were compared in 712 Ct-positive clinical samples. The Ct-MS assay showed a highly specific reaction for all probes with the amplicons of the reference strains, giving a very low background median fluorescence intensity signal (median fluorescence intensity ≤ 10). An excellent overall agreement in the Ct detection (kappa = 0.947, 95% confidence interval, 0.89 to 0.999; McNemar's test, P = 1.000) and the Ct genotyping (kappa = 0.993, 95% confidence interval, 0.977 to 1.000; McNemar's test, P = 0.053) was observed between the Ct detection and genoTyping (DT) assay and the Ct-MS assay. In conclusion, the novel Ct-MS assay permits simultaneous detection and genotyping of Ct serovars, making the Ct-MS assay an excellent high throughput method.Entities:
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Year: 2011 PMID： 21354049 PMCID： PMC3128579 DOI： 10.1016/j.jmoldx.2010.11.017
Source DB: PubMed Journal: J Mol Diagn ISSN： 1525-1578 Impact factor: 5.568