Literature DB >> 21353533

Effect of alkaline pre-treatment on enzyme synergy for efficient hemicellulose hydrolysis in sugarcane bagasse.

Natasha Beukes1, Brett I Pletschke.   

Abstract

This aim of this study was to investigate the effect of ammonium hydroxide (NH(4)OH) and sodium hydroxide (NaOH) pre-treatment on the digestibility of sugarcane bagasse (SCB) by hemicellulase action. It was found that pre-treatment of SCB with NH(4)OH removed a larger percentage of the SCB lignin and effectively increased SCB digestibility 13.13 fold. The greatest amount of reducing sugar (1194.88 μmol/min) and largest degree of synergy (2.85) was obtained using a combination of two enzymes (25% ManA and 75% XynA) with NH(4)OH pre-treated SCB. In this study, NH(4)OH therefore appeared to be a more effective pre-treatment step for subsequent hydrolysis by hemicellulases.
Copyright © 2011 Elsevier Ltd. All rights reserved.

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Year:  2011        PMID: 21353533     DOI: 10.1016/j.biortech.2011.01.090

Source DB:  PubMed          Journal:  Bioresour Technol        ISSN: 0960-8524            Impact factor:   9.642


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