Literature DB >> 21349102

Genetic analysis of ORF5 in porcine reproductive and respiratory syndrome virus in Japan.

Hiroshi Iseki1, Michihiro Takagi, Ayako Miyazaki, Ken Katsuda, Osamu Mikami, Hiroshi Tsunemitsu.   

Abstract

In recent years, no reports regarding genetic information on porcine reproductive and respiratory syndrome virus (PRRSV) with a focus on Japan have been published. To clarify the effect of time on PRRSV genomic evolution, we sequenced the open reading frame 5 (600 or 603 bases) obtained from Japanese PRRSV isolates for three periods (1992-1993, 2000-2001, and 2007-2008) and compared their phylogenetic relationships. Assessment of mean pairwise homology of nucleotide sequences of PRRSV isolates indicated a trend towards increasing heterogeneity over time. In addition, we newly detected a virus classified in cluster IV, indicative of the increasing genetic variation of PRRSV in Japan.
© 2011 The Societies and Blackwell Publishing Asia Pty Ltd.

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Year:  2011        PMID： 21349102     DOI： 10.1111/j.1348-0421.2010.00303.x

Source DB:  PubMed          Journal:  Microbiol Immunol        ISSN： 0385-5600            Impact factor:   1.955


  5 in total

1.  Application of a SYBR®Green one step real-time RT-PCR assay to detect type 1 porcine reproductive and respiratory syndrome virus.

Authors:  Hiroshi Iseki; Michihiro Takagi; Yoshiko Kuroda; Ken Katsuda; Osamu Mikami; Hiroshi Tsunemitsu; Makoto Yamakawa
Journal:  J Vet Med Sci       Date:  2014-07-18       Impact factor: 1.267

2.  Concise and broadly applicable method for determining the genomic sequences of North-American-type porcine reproductive and respiratory syndrome viruses in various clusters.

Authors:  Takeya Morozumi; Hiroshi Iseki; Daisuke Toki; Michihiro Takagi; Hiroshi Tsunemitsu; Hirohide Uenishi
Journal:  J Vet Med Sci       Date:  2014-06-10       Impact factor: 1.267

3.  Pathogenicity of emerging Japanese type 1 porcine reproductive and respiratory syndrome virus in experimentally infected pigs.

Authors:  Hiroshi Iseki; Michihiro Takagi; Kenji Kawashima; Tomoyuki Shibahara; Yoshiko Kuroda; Hiroshi Tsunemitsu; Makoto Yamakawa
Journal:  J Vet Med Sci       Date:  2015-07-05       Impact factor: 1.267

4.  Studies on heterologous protection between Japanese type 1 and type 2 porcine reproductive and respiratory syndrome virus isolates.

Authors:  Hiroshi Iseki; Kenji Kawashima; Michihiro Takagi; Tomoyuki Shibahara; Masaji Mase
Journal:  J Vet Med Sci       Date:  2020-05-22       Impact factor: 1.267

5.  Immunity against a Japanese local strain of porcine reproductive and respiratory syndrome virus decreases viremia and symptoms of a highly pathogenic strain.

Authors:  Hiroshi Iseki; Kenji Kawashima; Tomoyuki Shibahara; Masaji Mase
Journal:  BMC Vet Res       Date:  2021-04-13       Impact factor: 2.741
  5 in total

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