BACKGROUND: It has been suggested that the age of human organ donors might influence islet isolation and transplantation outcome in a negative way due to a decrease of in vivo function in islets isolated from older donors. METHODS: We retrospectively analyzed 332 islet isolations according to donor age. We determined isolation outcome by islet yields, transplantation rates, and [beta]-cell function in vitro. Transplanted patients were divided into two groups depending on donor age (n=25 and n=31 patients for <=45- and >45-year-old donors, respectively). We assessed islet graft function by C-peptide/glucose ratio, [beta] score, secretory units of islets in transplantation index, and insulin independence rate at 1, 6, and 12 months after transplantation. RESULTS: There was no difference in islet yields between the two groups (251,900+/-14,100 and 244,600+/-8400 islet equivalent for <=45- and >45-year-old donors, respectively). Transplantation rates and stimulation indices were similar in both groups as well. All islet graft function parameters were significantly higher at 1-month follow-up in patients who had received islets from younger donors. At 6-month follow-up after second or third injection and at 12-month follow-up, secretory units of islets in transplantation indices and C-peptide/glucose ratios were significantly higher in patients with donors aged 45 years or younger. CONCLUSIONS: These data suggest that, despite similar outcomes of the isolation procedure, islet graft function is significantly influenced by donor age. These results may have important consequences in the definition of pancreas allocation criteria.
BACKGROUND: It has been suggested that the age of human organ donors might influence islet isolation and transplantation outcome in a negative way due to a decrease of in vivo function in islets isolated from older donors. METHODS: We retrospectively analyzed 332 islet isolations according to donor age. We determined isolation outcome by islet yields, transplantation rates, and [beta]-cell function in vitro. Transplanted patients were divided into two groups depending on donor age (n=25 and n=31 patients for <=45- and >45-year-old donors, respectively). We assessed islet graft function by C-peptide/glucose ratio, [beta] score, secretory units of islets in transplantation index, and insulin independence rate at 1, 6, and 12 months after transplantation. RESULTS: There was no difference in islet yields between the two groups (251,900+/-14,100 and 244,600+/-8400 islet equivalent for <=45- and >45-year-old donors, respectively). Transplantation rates and stimulation indices were similar in both groups as well. All islet graft function parameters were significantly higher at 1-month follow-up in patients who had received islets from younger donors. At 6-month follow-up after second or third injection and at 12-month follow-up, secretory units of islets in transplantation indices and C-peptide/glucose ratios were significantly higher in patients with donors aged 45 years or younger. CONCLUSIONS: These data suggest that, despite similar outcomes of the isolation procedure, islet graft function is significantly influenced by donor age. These results may have important consequences in the definition of pancreas allocation criteria.
Authors: Barbora Voglová; Martina Zahradnická; Peter Girman; Jan Kríž; Zuzana Berková; Tomáš Koblas; Ema Vávrová; Lenka Németová; Lucie Kosinová; David Habart; Eva Fábryová; Eva Dovolilová; Ivan Leontovyc; Tomáš Neškudla; Jan Peregrin; Jozef Kovác; Kvetoslav Lipár; Matej Kocík; Tomáš Marada; Jirí Svoboda; František Saudek Journal: Rev Diabet Stud Date: 2017-06-12
Authors: A N Balamurugan; Gopalakrishnan Loganathan; Melena D Bellin; Joshua J Wilhelm; James Harmon; Takayuki Anazawa; Sajjad M Soltani; David M Radosevich; Takeshi Yuasa; Mukesh Tiwari; Klearchos K Papas; Robert McCarthy; David E R Sutherland; Bernhard J Hering Journal: Transplantation Date: 2012-04-15 Impact factor: 4.939
Authors: Wilson Km Wong; Guozhi Jiang; Anja E Sørensen; Yi Vee Chew; Cody Lee-Maynard; David Liuwantara; Lindy Williams; Philip J O'Connell; Louise T Dalgaard; Ronald C Ma; Wayne J Hawthorne; Mugdha V Joglekar; Anandwardhan A Hardikar Journal: JCI Insight Date: 2019-07-30
Authors: Ling-Jia Wang; Tatsuya Kin; Doug O'Gorman; A M James Shapiro; Bashoo Naziruddin; Morihito Takita; Marlon F Levy; Andrew M Posselt; Gregory L Szot; Omid Savari; Barbara Barbaro; James McGarrigle; Chun Chieh Yeh; Jose Oberholzer; Ji Lei; Tao Chen; Moh Lian; James F Markmann; Alejandro Alvarez; Elina Linetsky; Camillo Ricordi; A N Balamurugan; Gopalakrishnan Loganathan; Joshua J Wilhelm; Bernhard J Hering; Rita Bottino; Massimo Trucco; Chengyang Liu; Zaw Min; Yanjing Li; Ali Naji; Luis A Fernandez; Martynas Ziemelis; Juan S Danobeitia; J Michael Millis; Piotr Witkowski Journal: Cell Transplant Date: 2016-02-26 Impact factor: 4.064
Authors: Meirigeng Qi; Luis Valiente; Brian McFadden; Keiko Omori; Shiela Bilbao; Jemily Juan; Jeffrey Rawson; Stephen Scott; Kevin Ferreri; Yoko Mullen; Mohamed El-Shahawy; Donald Dafoe; Fouad Kandeel; Ismail H Al-Abdullah Journal: Transplant Direct Date: 2015-05