Literature DB >> 21340564

Immunoblot diagnosis of infection with Neospora caninum in cattle based on recombinant NcSAG4 Antigen.

Jinqiang Hu1, Ezio Ferroglio, Anna Trisciuoglio.   

Abstract

The Neospora caninum (N. caninum) NcSAG4 gene was subcloned into a pET-28a (+) vector and successfully expressed in Escherichia coli as inclusion body, and confirmed by SDS-PAGE and Western blot using anti-His monoclonal antibody. The purified protein was then purified using Ni-NTA affinity chromatography column and recognized by positive serum from N. caninum-infected cattle. Immunoblot (IB) method based on purified recombinant NcSAG4 (rNcSAG4) antigen to detect antibodies against N. caninum in cattle was developed. Subsequently, both IB and ELISA kit were used to test sera (52) from naturally infected/uninfected cattle. Results showed that 50 and 48 out of 52 was positive for IB and ELISA kit, respectively, revealing that IB is more or at least as sensitive as ELISA when used for serodiagnosis of infected cattle.

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Year:  2011        PMID： 21340564     DOI： 10.1007/s00436-011-2286-7

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  15 in total

Review 1.  Neospora caninum: a cause of immune-mediated failure of pregnancy?

Authors:  Helen E Quinn; John T Ellis; Nicholas C Smith
Journal:  Trends Parasitol       Date:  2002-09

Review 2.  A review of Neospora caninum and neosporosis.

Authors:  J P Dubey; D S Lindsay
Journal:  Vet Parasitol       Date:  1996-12-02       Impact factor: 2.738

3.  Comparison of intracerebral parasite load, lesion development, and systemic cytokines in mouse strains infected with Neospora caninum.

Authors:  M T Long; T V Baszler; B A Mathison
Journal:  J Parasitol       Date:  1998-04       Impact factor: 1.276

4.  Sensitive and specific identification of Neospora caninum infection of cattle based on detection of serum antibodies to recombinant Ncp29.

Authors:  Daniel K Howe; Keliang Tang; Patricia A Conrad; Karen Sverlow; J P Dubey; L David Sibley
Journal:  Clin Diagn Lab Immunol       Date:  2002-05

5.  Immunization with native surface protein NcSRS2 induces a Th2 immune response and reduces congenital Neospora caninum transmission in mice.

Authors:  G J Haldorson; B A Mathison; K Wenberg; P A Conrad; J P Dubey; A J Trees; I Yamane; T V Baszler
Journal:  Int J Parasitol       Date:  2005-07-18       Impact factor: 3.981

6.  Prevalence and transmission of Neospora caninum within infected Swedish dairy herds.

Authors:  Jenny Frössling; Arvid Uggla; Camilla Björkman
Journal:  Vet Parasitol       Date:  2005-01-25       Impact factor: 2.738

7.  Redescription of Neospora caninum and its differentiation from related coccidia.

Authors:  J P Dubey; B C Barr; J R Barta; I Bjerkås; C Björkman; B L Blagburn; D D Bowman; D Buxton; J T Ellis; B Gottstein; A Hemphill; D E Hill; D K Howe; M C Jenkins; Y Kobayashi; B Koudela; A E Marsh; J G Mattsson; M M McAllister; D Modrý; Y Omata; L D Sibley; C A Speer; A J Trees; A Uggla; S J Upton; D J L Williams; D S Lindsay
Journal:  Int J Parasitol       Date:  2002-07       Impact factor: 3.981

8.  Specific anti-Neospora caninum IgG1 and IgG2 antibody responses during gestation in naturally infected cattle and their relationship with gamma interferon production.

Authors:  S Almería; C Nogareda; P Santolaria; I Garcia-Ispierto; J L Yániz; F López-Gatius
Journal:  Vet Immunol Immunopathol       Date:  2009-01-19       Impact factor: 2.046

9.  Detection of serum antibody responses in cattle with natural or experimental Neospora infections.

Authors:  P A Conrad; K Sverlow; M Anderson; J Rowe; R BonDurant; G Tuter; R Breitmeyer; C Palmer; M Thurmond; A Ardans
Journal:  J Vet Diagn Invest       Date:  1993-10       Impact factor: 1.279

10.  A multiple antigen ELISA to detect Neospora-specific antibodies in bovine sera, bovine foetal fluids, ovine and caprine sera.

Authors:  T Osawa; J Wastling; S Maley; D Buxton; E A Innes
Journal:  Vet Parasitol       Date:  1998-09       Impact factor: 2.738
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  4 in total

1.  Neospora caninum surface antigen (p40) is a potential diagnostic marker for cattle neosporosis.

Authors:  Pengfei He; Jianhua Li; Pengtao Gong; Chengwu Liu; Guocai Zhang; Ju Yang; Wenbin Tuo; Bintong Yang; Xichen Zhang
Journal:  Parasitol Res       Date:  2013-02-23       Impact factor: 2.289

2.  Chimeric Protein Designed by Genome-Scale Immunoinformatics Enhances Serodiagnosis of Bovine Neosporosis.

Authors:  Higor Sette Pereira; Ludmila Tavares E Almeida; Vitória Fernandes; Renato Lima Senra; Patrícia Pereira Fontes; Eustáquio Resende Bittar; Andréa de Oliveira Barros Ribon; Polyana Pizzi Rotta; Daniel Menezes-Souza; Joely Ferreira Figueiredo Bittar; Tiago Antônio de Oliveira Mendes
Journal:  J Clin Microbiol       Date:  2020-06-24       Impact factor: 5.948

3.  Comparative study of protective activities of Neospora caninum bradyzoite antigens, NcBAG1, NcBSR4, NcMAG1, and NcSAG4, in a mouse model of acute parasitic infection.

Authors:  Masaki Uchida; Kotomi Nagashima; Yui Akatsuka; Takashi Murakami; Akira Ito; Soichi Imai; Kazunori Ike
Journal:  Parasitol Res       Date:  2012-11-15       Impact factor: 2.289

4.  Development and Standardization of Dot - ELISA for Detection of Neospora caninum Antibodies in Cattle and Comparison with Standard Indirect ELISA and Direct Agglutination Test (DAT).

Authors:  Hossein Hamidinejat; Mohamad Rahim Haji Hajikolaei; Masoud Ghorbanpoor; Mehdi Namavari; Sara Mohamad Ali Gol
Journal:  Iran J Parasitol       Date:  2013-10       Impact factor: 1.012
  4 in total

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