PURPOSE: To detect somatic cytogenetic abnormalities and AZF microdeletions in a sample of 187 Turkish infertile men to determine the frequencies and the characteristics of our primary male infertility data in order to perform appropriate genetic counseling. METHODS: This study included 187 infertile men. Chromosomal studies and screening of AZF deletions was performed by multiplex polymerase chain reaction (PCR) analysis using the Y Chromosome Deletion Detection System. RESULTS: Cytogenetic study revealed chromosomal abnormality in 9 subjects (4.8%). In remaining 178 subjects, 7 subjects (3.93%) were detected to have Y chromosome microdeletions. The AZFc region was the most frequently involved region in microdeletion process in affected subjects. All subjects having microdeletion were azoospermic CONCLUSIONS: Cytogenetic and molecular study should be performed to obtain reliable genetic information for the genetic counseling of primary infertile man. Y chromosome microdeletion diagnosis is useful in decision making for assisted reproductive technics.
PURPOSE: To detect somatic cytogenetic abnormalities and AZF microdeletions in a sample of 187 Turkish infertile men to determine the frequencies and the characteristics of our primary male infertility data in order to perform appropriate genetic counseling. METHODS: This study included 187 infertile men. Chromosomal studies and screening of AZF deletions was performed by multiplex polymerase chain reaction (PCR) analysis using the Y Chromosome Deletion Detection System. RESULTS: Cytogenetic study revealed chromosomal abnormality in 9 subjects (4.8%). In remaining 178 subjects, 7 subjects (3.93%) were detected to have Y chromosome microdeletions. The AZFc region was the most frequently involved region in microdeletion process in affected subjects. All subjects having microdeletion were azoospermic CONCLUSIONS: Cytogenetic and molecular study should be performed to obtain reliable genetic information for the genetic counseling of primary infertile man. Y chromosome microdeletion diagnosis is useful in decision making for assisted reproductive technics.
Authors: J H Tuerlings; H F de France; A Hamers; R Hordijk; J O Van Hemel; K Hansson; J M Hoovers; K Madan; M Van der Blij-Philipsen; K B Gerssen-Schoorl; J A Kremer; D F Smeets Journal: Eur J Hum Genet Date: 1998 May-Jun Impact factor: 4.246
Authors: K van der Ven; M Montag; B Peschka; J Leygraaf; G Schwanitz; G Haidl; D Krebs; H van der Ven Journal: Mol Hum Reprod Date: 1997-08 Impact factor: 4.025
Authors: P H Vogt; A Edelmann; S Kirsch; O Henegariu; P Hirschmann; F Kiesewetter; F M Köhn; W B Schill; S Farah; C Ramos; M Hartmann; W Hartschuh; D Meschede; H M Behre; A Castel; E Nieschlag; W Weidner; H J Gröne; A Jung; W Engel; G Haidl Journal: Hum Mol Genet Date: 1996-07 Impact factor: 6.150
Authors: Taha Reşid Özdemir; Berk Özyılmaz; Özgür Çakmak; Özge Özer Kaya; Can Köse; Özgür Kırbıyık; Mehmet Zeynel Keskin; Altuğ Koç; Tuğba Zeyrek; Yaşar Bekir Kutbay; Kadri Murat Erdoğan; Merve Saka Güvenç Journal: Turk J Urol Date: 2019-11-29