| Literature DB >> 21337254 |
Federica Menichini1, Rosa Tundis, Marco Bonesi, Bruno de Cindio, Monica R Loizzo, Filomena Conforti, Giancarlo A Statti, Roberta Menabeni, Ruggero Bettini, Francesco Menichini.
Abstract
The chemical composition of the essential oil of Citrus medica L. cv. Diamante peel obtained by hydrodistillation, cold-pressing and supercritical carbon dioxide extraction techniques was determined by GC/MS analysis. Forty-six components were fully characterised. Limonene and γ-terpinene were the major components of the oils obtained by hydrodistillation (HD) and cold-pressing (CP), while citropten was the major constituent in the oil obtained by supercritical carbon dioxide extraction (SFE). Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities were evaluated. The essential oil obtained by hydrodistillation exerted the highest inhibitory activity against BChE (IC₅₀ value of 154.6 µg mL⁻¹) and AChE (IC₅₀ value of 171.3 µg mL⁻¹. Interestingly, the oil obtained by cold-pressing exhibited a selective inhibitory activity against AChE. The essential oils have also been evaluated for the inhibition of NO production in LPS induced RAW 264.7 macrophages. The oil obtained by hydrodistillation exerted a significant inhibition of NO production with an IC₅₀ value of 17 µg mL⁻¹ (IC₅₀ of positive control 53 µg mL⁻¹).Entities:
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Year: 2011 PMID： 21337254 DOI： 10.1080/14786410902900085
Source DB: PubMed Journal: Nat Prod Res ISSN： 1478-6419 Impact factor: 2.861