PURPOSE: Organ transplantation necessitates the use of preservation solutions to maintain the integrity of the organs during retrieval. The aim of this study was to investigate the flow distribution in abdominal organs in rats during acute infusion of preservation solution. METHODS: Microspheres were used to estimate the distribution of flow in the pancreas, duodenum, ileum, colon, liver, kidneys and lungs in untreated Wistar-Furth rats and in animals with an opened abdominal cavity and catheterised aorta. Some animals were infused by free flow of 5 ml of UW, HTK or Ringer solution containing microspheres at a pressure of 100 cm H(2)O through an intra-aortic catheter. RESULTS: Opening of the abdominal cavity did not affect any of the organ blood flow values. However, the fraction of total pancreatic blood flow diverted through the islets increased. During infusion of microsphere-containing UW, HTK or Ringer solution, splanchnic and renal organ flow values, represented by microsphere contents, were similar. The fraction of microspheres found in the islets was lower in UW-infused rats. The number of microspheres present in the lungs or liver was very low, suggesting that shunting was negligible. CONCLUSIONS: Infusion of HTK and UW solution into anaesthetised rats results in a flow distribution which is similar to that in normal animals in most abdominal organs, but there is a reduction in islet blood perfusion by UW but not HTK solution.
PURPOSE: Organ transplantation necessitates the use of preservation solutions to maintain the integrity of the organs during retrieval. The aim of this study was to investigate the flow distribution in abdominal organs in rats during acute infusion of preservation solution. METHODS: Microspheres were used to estimate the distribution of flow in the pancreas, duodenum, ileum, colon, liver, kidneys and lungs in untreated Wistar-Furth rats and in animals with an opened abdominal cavity and catheterised aorta. Some animals were infused by free flow of 5 ml of UW, HTK or Ringer solution containing microspheres at a pressure of 100 cm H(2)O through an intra-aortic catheter. RESULTS: Opening of the abdominal cavity did not affect any of the organ blood flow values. However, the fraction of total pancreatic blood flow diverted through the islets increased. During infusion of microsphere-containing UW, HTK or Ringer solution, splanchnic and renal organ flow values, represented by microsphere contents, were similar. The fraction of microspheres found in the islets was lower in UW-infused rats. The number of microspheres present in the lungs or liver was very low, suggesting that shunting was negligible. CONCLUSIONS: Infusion of HTK and UW solution into anaesthetised rats results in a flow distribution which is similar to that in normal animals in most abdominal organs, but there is a reduction in islet blood perfusion by UW but not HTK solution.
Authors: J Bitu-Moreno; I Francischetti; R Siemer; G Matheis; R Baretti; F H Maffei; B Kreitmayr; F Beyersdorf Journal: Eur J Cardiothorac Surg Date: 1999-04 Impact factor: 4.191
Authors: F C Brunicardi; J Stagner; S Bonner-Weir; H Wayland; R Kleinman; E Livingston; P Guth; M Menger; R McCuskey; M Intaglietta; A Charles; S Ashley; A Cheung; E Ipp; S Gilman; T Howard; E Passaro Journal: Diabetes Date: 1996-04 Impact factor: 9.461
Authors: Dirk Uhlmann; Barbara Armann; Stefan Ludwig; Evelyn Escher; Uta-Carolin Pietsch; Andrea Tannapfel; Daniel Teupser; Johann Hauss; Helmut Witzigmann Journal: J Surg Res Date: 2002-06-15 Impact factor: 2.192
Authors: U Leonhardt; A Tytko; B Exner; M Barthel; F Stöckmann; H Köhler; E G Siegel; K Nebendahl; W Creutzfeldt Journal: Transplantation Date: 1993-01 Impact factor: 4.939
Authors: Daniel Foltys; Moritz Kaths; Mari Strempel; Uwe Scheuermann; Axel Heimann; Veronika Weyer; Torsten Hansen; Oliver Kempski; Gerd Otto Journal: Transplant Res Date: 2013-07-09