OBJECTIVES: The aim of this work was to study the tick-borne encephalitis virus (TBEV) infection of goats and the possibilities to prevent human milk-borne infections either by immunizing animals or the heat treatment of milk. METHODS: An experiment was conducted with 20 milking goats. Ten goats (half of them immunized) were challenged with live TBEV and 10 were left uninfected. Clinical signs and body temperatures of the animals were recorded and milk samples were collected daily. The presence of viral RNA and infectious virions in milk were detected by RT-PCR and intracerebral inoculation of suckling mice, respectively. Milk samples containing infectious virions were subjected to various heat treatment conditions and retested afterwards to assess the effect on infectivity. RESULTS: The infected goats did not show any clinical signs or fever compared to uninfected ones. Infectious virions were detected for 8-19 days from the milk samples (genome for 3-18 days by PCR) of infected goats. Immunized goats did not shed the virus. After heat treatment of the milk, the inoculated mice survived. CONCLUSIONS: Goats shed the virus with their milk without showing any symptoms. Human milk-borne infections can be avoided both by immunizing goats and boiling/pasteurizing infected milk.
OBJECTIVES: The aim of this work was to study the tick-borne encephalitis virus (TBEV) infection of goats and the possibilities to prevent humanmilk-borne infections either by immunizing animals or the heat treatment of milk. METHODS: An experiment was conducted with 20 milking goats. Ten goats (half of them immunized) were challenged with live TBEV and 10 were left uninfected. Clinical signs and body temperatures of the animals were recorded and milk samples were collected daily. The presence of viral RNA and infectious virions in milk were detected by RT-PCR and intracerebral inoculation of suckling mice, respectively. Milk samples containing infectious virions were subjected to various heat treatment conditions and retested afterwards to assess the effect on infectivity. RESULTS: The infected goats did not show any clinical signs or fever compared to uninfected ones. Infectious virions were detected for 8-19 days from the milk samples (genome for 3-18 days by PCR) of infected goats. Immunized goats did not shed the virus. After heat treatment of the milk, the inoculated mice survived. CONCLUSIONS:Goats shed the virus with their milk without showing any symptoms. Humanmilk-borne infections can be avoided both by immunizing goats and boiling/pasteurizing infected milk.
Authors: Leo Markovinović; M L Kosanović Ličina; V Tešić; D Vojvodić; I Vladušić Lucić; T Kniewald; T Vukas; M Kutleša; Lidija Cvetko Krajinović Journal: Infection Date: 2016-06-30 Impact factor: 3.553
Authors: Dace Zavadska; Ioana Anca; Francis André; Mustafa Bakir; Roman Chlibek; Milan Cižman; Inga Ivaskeviciene; Atanas Mangarov; Zsófia Mészner; Marko Pokorn; Roman Prymula; Darko Richter; Nuran Salman; Pavol Simurka; Eda Tamm; Goran Tešović; Ingrid Urbancikova; Vytautas Usonis Journal: Hum Vaccin Immunother Date: 2013-01-04 Impact factor: 3.452
Authors: Brigitte Böhm; Benjamin Schade; Benjamin Bauer; Bernd Hoffmann; Donata Hoffmann; Ute Ziegler; Martin Beer; Christine Klaus; Herbert Weissenböck; Jens Böttcher Journal: BMC Vet Res Date: 2017-08-22 Impact factor: 2.741
Authors: Brigitta Zana; Károly Erdélyi; Anna Nagy; Eszter Mezei; Orsolya Nagy; Mária Takács; Tamás Bakonyi; Petra Forgách; Orsolya Korbacska-Kutasi; Orsolya Fehér; Péter Malik; Krisztina Ursu; Péter Kertész; Anett Kepner; Máté Martina; Tamás Süli; Zsófia Lanszki; Gábor Endre Tóth; Anett Kuczmog; Balázs Somogyi; Ferenc Jakab; Gábor Kemenesi Journal: Viruses Date: 2020-01-20 Impact factor: 5.048