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Literature DB >> 21324348

Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua.

Trond Løvdal¹, Maria Befring Hovda, Benny Björkblom, Simon G Møller.

Abstract

The combination of propidium monoazide (PMA) and quantitative real-time PCR (qPCR) significantly overestimated the fraction of viable Listeria innocua as compared to plate counts and confocal fluorescence microscopy. Our data imply that PMA-qPCR must be used with caution as an analytical tool for the differentiation between viable and dead bacteria.

Entities: Chemical Species

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Year: 2011 PMID： 21324348 DOI： 10.1016/j.mimet.2011.01.027

Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363

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Cited

23 in total

1. Viability Quantitative PCR Utilizing Propidium Monoazide, Spheroplast Formation, and Campylobacter coli as a Bacterial Model.

Authors: Thomai P Lazou; Eleni G Iossifidou; Athanasios I Gelasakis; Serafeim C Chaintoutis; Chrysostomos I Dovas
Journal: Appl Environ Microbiol Date: 2019-10-01 Impact factor: 4.792

Review 2. Dead or alive: molecular assessment of microbial viability.

Authors: Gerard A Cangelosi; John S Meschke
Journal: Appl Environ Microbiol Date: 2014-07-18 Impact factor: 4.792

3. Molecular Viability Testing of UV-Inactivated Bacteria.

Authors: Kris M Weigel; Felicia K Nguyen; Moira R Kearney; John S Meschke; Gerard A Cangelosi
Journal: Appl Environ Microbiol Date: 2017-05-01 Impact factor: 4.792

4. Detection and Quantification of Viable and Nonviable Trypanosoma cruzi Parasites by a Propidium Monoazide Real-Time Polymerase Chain Reaction Assay.

Authors: Beatriz Cancino-Faure; Roser Fisa; M Magdalena Alcover; Teresa Jimenez-Marco; Cristina Riera
Journal: Am J Trop Med Hyg Date: 2016-05-02 Impact factor: 2.345

5. Experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents.

Authors: Jérémy Desneux; Marianne Chemaly; Anne-Marie Pourcher
Journal: BMC Microbiol Date: 2015-08-16 Impact factor: 3.605

6. Species-specific viability analysis of Pseudomonas aeruginosa, Burkholderia cepacia and Staphylococcus aureus in mixed culture by flow cytometry.

Authors: Marc Rüger; Mandy Ackermann; Udo Reichl
Journal: BMC Microbiol Date: 2014-03-07 Impact factor: 3.605

Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua.

1. Viability Quantitative PCR Utilizing Propidium Monoazide, Spheroplast Formation, and Campylobacter coli as a Bacterial Model.

Review 2. Dead or alive: molecular assessment of microbial viability.

3. Molecular Viability Testing of UV-Inactivated Bacteria.

4. Detection and Quantification of Viable and Nonviable Trypanosoma cruzi Parasites by a Propidium Monoazide Real-Time Polymerase Chain Reaction Assay.

5. Experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents.

6. Species-specific viability analysis of Pseudomonas aeruginosa, Burkholderia cepacia and Staphylococcus aureus in mixed culture by flow cytometry.

7. Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide.

Review 8. Pathogens protection against the action of disinfectants in multispecies biofilms.

9. Biosynthetic enhancement of the detection of bacteria by the polymerase chain reaction.

10. Assessments of total and viable Escherichia coli O157:H7 on field and laboratory grown lettuce.