Literature DB >> 21324

Detection of circulating immune complexes with a modified Raji cell technique.

T H Hütteroth, K H Meyer zum Büschenfelde.   

Abstract

A modification of the Raji cell technique for the detection of circulating immune complexes is described. Raji lymphoid cells bind immune complexes via the C 3 receptor. Prior to incubation with the test serum it is necessary to block the IgG Fc receptor which would interfere with the assay. In the present modification the IgG Fc receptor is blocked with monoclonal IgG myeloma proteins thereby facilitating the detection of C 3 bound immune complexes with fluoresecein-conjugated anti-light chain serum. This modification is presently used in a survey of immune complexes in a number of autoimmune diseases.

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Year:  1977        PMID： 21324     DOI： 10.1007/BF01478825

Source DB:  PubMed          Journal:  Klin Wochenschr        ISSN： 0023-2173


  12 in total

Review 1.  Immune complex disease in experimental animals and man.

Authors:  C G Cochrane; D Koffler
Journal:  Adv Immunol       Date:  1973       Impact factor: 3.543

2.  Circulating immune complexes in dermatitis herpetiformis.

Authors:  J F Mowbray; A V Hoffbrand; E J Holborow; P P Seah; L Fry
Journal:  Lancet       Date:  1973-02-24       Impact factor: 79.321

3.  Cultured lymphoid cell lines from normal subjects: membrane associated immunoglobulins studied by the mixed antiglobulin reaction.

Authors:  T H Hütteroth; S D Litwin; H Cleve
Journal:  Cell Immunol       Date:  1972-11       Impact factor: 4.868

4.  Biologic and clinical significance of cryoglobulins. A report of 86 cases.

Authors:  J C Brouet; J P Clauvel; F Danon; M Klein; M Seligmann
Journal:  Am J Med       Date:  1974-11       Impact factor: 4.965

5.  The Raji cell radioimmune assay for detecting immune complexes in human sera.

Authors:  A N Theofilopoulos; C B Wilson; F J Dixon
Journal:  J Clin Invest       Date:  1976-01       Impact factor: 14.808

6.  Circulating immune complexes in the serum in systemic lupus erythematosus and in carriers of hepatitis B antigen. Quantitation by binding to radiolabeled C1q.

Authors:  U E Nydegger; P H Lambert; H Gerber; P A Miescher
Journal:  J Clin Invest       Date:  1974-08       Impact factor: 14.808

7.  Binding of components of the properdin system to cultured human lymphoblastoid cells and B lymphocytes.

Authors:  A N Theofilopoulos; L H Perrin
Journal:  J Exp Med       Date:  1976-02-01       Impact factor: 14.307

8.  C1q deviation test for the detection of immune complexes, aggregates of IgG, and bacterial products in human serum.

Authors:  A T Sobel; V A Bokisch; H J Müller-Eberhard
Journal:  J Exp Med       Date:  1975-07-01       Impact factor: 14.307

9.  An unusual protein component of high molecular weight in the serum of certain patients with rheumatoid arthritis.

Authors:  E C FRANKLIN; H R HOLMAN; H J MULLER-EBERHARD; H G KUNKEL
Journal:  J Exp Med       Date:  1957-05-01       Impact factor: 14.307

10.  Binding of soluble immune complexes to human lymphoblastoid cells. II. Use of Raji cells to detect circulating immune complexes in animal and human sera.

Authors:  A N Theofilopoulos; C B Wilson; V A Bokisch; F J Dixon
Journal:  J Exp Med       Date:  1974-11-01       Impact factor: 14.307
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  2 in total

1.  Antigenic relationship between Chang liver cells and human hepatocytes.

Authors:  T H Hütteroth; K H Meyer
Journal:  Klin Wochenschr       Date:  1978-05-15

2.  Spontaneous cell-mediated (SCMC) and antibody-dependent cellular cytotoxicity (ADCC) in patients with acute and chronic active hepatitis.

Authors:  T H Hütteroth; T Poralla; K H Meyer zum Büschenfelde
Journal:  Klin Wochenschr       Date:  1981-06-15
  2 in total

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