AIMS: This study has compared the tissue expression of the p53 tumour suppressor protein and DNA repair proteins APE1, hMSH2 and ERCC1 in normal, dysplastic and malignant lip epithelium. METHODS AND RESULTS: Morphological analysis and immunohistochemistry were performed on archived specimens of normal lip mucosa (n=15), actinic cheilitis (AC) (n=30), and lip squamous cell carcinoma (LSCC) (n=27). AC samples were classified morphologically according to the severity of epithelial dysplasia and risk of malignant transformation. LSCC samples were morphologically staged according to WHO and invasive front grading (IFG) criteria. Differences between groups and morphological stages were determined by bivariate statistical analysis. Progressive increases in the percentage of epithelial cells expressing p53 and APE1 were associated with increases in morphological malignancy from normal lip mucosa to LSCC. There was also a significant reduction in epithelial cells expressing hMSH2 and ERCC1 proteins in the AC and LSCC groups. A higher percentage of malignant cells expressing APE1 was found in samples with an aggressive morphological IFG grade. CONCLUSIONS: Our data showed that epithelial cells from premalignant to malignant lip disease exhibited changes in the expression of p53, APE1, hMSH2 and ERCC1 proteins; these molecular change might contribute to lip carcinogenesis.
AIMS: This study has compared the tissue expression of the p53tumour suppressor protein and DNA repair proteins APE1, hMSH2 and ERCC1 in normal, dysplastic and malignant lip epithelium. METHODS AND RESULTS: Morphological analysis and immunohistochemistry were performed on archived specimens of normal lip mucosa (n=15), actinic cheilitis (AC) (n=30), and lip squamous cell carcinoma (LSCC) (n=27). AC samples were classified morphologically according to the severity of epithelial dysplasia and risk of malignant transformation. LSCC samples were morphologically staged according to WHO and invasive front grading (IFG) criteria. Differences between groups and morphological stages were determined by bivariate statistical analysis. Progressive increases in the percentage of epithelial cells expressing p53 and APE1 were associated with increases in morphological malignancy from normal lip mucosa to LSCC. There was also a significant reduction in epithelial cells expressing hMSH2 and ERCC1 proteins in the AC and LSCC groups. A higher percentage of malignant cells expressing APE1 was found in samples with an aggressive morphological IFG grade. CONCLUSIONS: Our data showed that epithelial cells from premalignant to malignant lip disease exhibited changes in the expression of p53, APE1, hMSH2 and ERCC1 proteins; these molecular change might contribute to lip carcinogenesis.
Authors: Lilian Mendes Borburema Cangussu; Ludmilla Regina de Souza; Marcela Gonçalves de Souza; Renato Sobral Monteiro Junior; Luis Alexandre Muehlmann; Paulo Narcizo de Souza; Lucyana Conceição Farias; Sérgio Henrique Sousa Santos; Alfredo Maurício Batista de Paula; André Luiz Sena Guimarães Journal: Lasers Med Sci Date: 2022-02-04 Impact factor: 3.161
Authors: Maria Luiza Diniz de Sousa Lopes; Francisco Leonardo da Silva Júnior; Kenio Costa Lima; Patrícia Teixeira de Oliveira; Éricka Janine Dantas da Silveira Journal: An Bras Dermatol Date: 2015 Jul-Aug Impact factor: 1.896
Authors: Dmitry José de Santana Sarmento; Gustavo Pina Godoy; Márcia Cristina da Costa Miguel; Éricka Janine Dantas da Silveira Journal: An Bras Dermatol Date: 2016 Jul-Aug Impact factor: 1.896