OBJECTIVE: Over the past 3 years, numerous outbreaks of infectious laryngotracheitis (ILT) have occurred in poultry in Australia. The objectives of this study were to identify the viral strains involved in the recent outbreaks and to determine possible epidemiological links between these outbreaks. PROCEDURE: A combination of polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analyses of several genes of the ILT virus was used to identify genetic differences in field/vaccine ILT virus isolates. In a previous study, these procedures had demonstrated five classes (1-5) in Australia. RESULTS: Analysis of 92 field ILT viruses demonstrated four new classes: 6, 7, 8 and 9. Class 6 was responsible for four outbreaks in one Victorian broiler company and demonstrated to be distinct from other Australian strains of ILT. Class 7 was the Nobilis ILT vaccine (Intervet Pty Ltd). Class 8 was responsible for the majority of the outbreaks in New South Wales and was phylogenetically close to class 7. On one occasion, classes 7 and 8 were identified in an outbreak on a Victorian farm that had used the Nobilis ILT vaccine. Class 9, also phylogenetically close to classes 7 and 8, was found only in New South Wales. The previously identified class 2 was also found to be responsible for a large number of outbreaks, mainly in Victoria. CONCLUSION: The results demonstrate that, epidemiologically, most outbreaks of ILT in New South Wales are unrelated to those in Victoria and suggest a link between classes 8 and 9 and the Nobilis ILT vaccine (class 7).
OBJECTIVE: Over the past 3 years, numerous outbreaks of infectious laryngotracheitis (ILT) have occurred in poultry in Australia. The objectives of this study were to identify the viral strains involved in the recent outbreaks and to determine possible epidemiological links between these outbreaks. PROCEDURE: A combination of polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analyses of several genes of the ILT virus was used to identify genetic differences in field/vaccine ILT virus isolates. In a previous study, these procedures had demonstrated five classes (1-5) in Australia. RESULTS: Analysis of 92 field ILT viruses demonstrated four new classes: 6, 7, 8 and 9. Class 6 was responsible for four outbreaks in one Victorian broiler company and demonstrated to be distinct from other Australian strains of ILT. Class 7 was the Nobilis ILT vaccine (Intervet Pty Ltd). Class 8 was responsible for the majority of the outbreaks in New South Wales and was phylogenetically close to class 7. On one occasion, classes 7 and 8 were identified in an outbreak on a Victorian farm that had used the Nobilis ILT vaccine. Class 9, also phylogenetically close to classes 7 and 8, was found only in New South Wales. The previously identified class 2 was also found to be responsible for a large number of outbreaks, mainly in Victoria. CONCLUSION: The results demonstrate that, epidemiologically, most outbreaks of ILT in New South Wales are unrelated to those in Victoria and suggest a link between classes 8 and 9 and the Nobilis ILT vaccine (class 7).
Authors: Peter Norberg; Daniel P Depledge; Samit Kundu; Claire Atkinson; Julianne Brown; Tanzina Haque; Yusuf Hussaini; Eithne MacMahon; Pamela Molyneaux; Vassiliki Papaevangelou; Nitu Sengupta; Evelyn S C Koay; Julian W Tang; Gillian S Underhill; Anna Grahn; Marie Studahl; Judith Breuer; Tomas Bergström Journal: J Virol Date: 2015-07 Impact factor: 6.549
Authors: Sang-Won Lee; Philip F Markham; John F Markham; Ivonne Petermann; Amir H Noormohammadi; Glenn F Browning; Nino P Ficorilli; Carol A Hartley; Joanne M Devlin Journal: BMC Genomics Date: 2011-04-19 Impact factor: 3.969
Authors: Sang-Won Lee; Carol A Hartley; Mauricio J C Coppo; Paola K Vaz; Alistair R Legione; José A Quinteros; Amir H Noormohammadi; Phillip F Markham; Glenn F Browning; Joanne M Devlin Journal: PLoS One Date: 2015-03-18 Impact factor: 3.240
Authors: Sang-Won Lee; Joanne M Devlin; John F Markham; Amir H Noormohammadi; Glenn F Browning; Nino P Ficorilli; Carol A Hartley; Philip F Markham Journal: PLoS One Date: 2013-02-01 Impact factor: 3.240
Authors: Mesula G Korsa; Glenn F Browning; Mauricio J C Coppo; Alistair R Legione; James R Gilkerson; Amir H Noormohammadi; Paola K Vaz; Sang-Won Lee; Joanne M Devlin; Carol A Hartley Journal: PLoS One Date: 2015-09-14 Impact factor: 3.240