BACKGROUND: Stored vascular tissues are employed in biomedical research for studies in imaging, in biomechanics, and/or in assessing vessel diseases. In the present study, the stability of aortic tissue in phosphate buffer saline (PBS) at 4°C was monitored over a course of 10 days as determined by the rate of glucose permeation measured by optical coherence tomography (OCT) and validated by histology. METHODS AND RESULTS: The initial mean permeability through fresh porcine aorta was (2.32 ± 0.46)× 10(-5)cm/s (n=5); after maintaining the tissue at 4°C for 10 days, the mean rate was (7.37 ± 0.41)× 10(-5)cm/s (n=4), an increase of nearly 300%. A z-test verified that a significant change in the permeability rate (p<0.05) had occurred after 4 days of 4°C storage. Histology was used to quantify changes in tissue pore area. The increase in average pore area paralleled the increase in permeability rate over 10 days. CONCLUSIONS: These results suggest that (1) the structural integrity of aortic tissue at 4°C is retained for at least the first three days after resection and (2) OCT is a powerful technology well suited for evaluating tissue structural integrity over time. GENERAL SIGNIFICANCE: Functional OCT imaging provides for a noninvasive and quantitative technique in determining the structural integrity of aortic tissue stored at 4°C. This modality may be used for assessing the efficacy of other preservation techniques.
BACKGROUND: Stored vascular tissues are employed in biomedical research for studies in imaging, in biomechanics, and/or in assessing vessel diseases. In the present study, the stability of aortic tissue in phosphate buffer saline (PBS) at 4°C was monitored over a course of 10 days as determined by the rate of glucose permeation measured by optical coherence tomography (OCT) and validated by histology. METHODS AND RESULTS: The initial mean permeability through fresh porcine aorta was (2.32 ± 0.46)× 10(-5)cm/s (n=5); after maintaining the tissue at 4°C for 10 days, the mean rate was (7.37 ± 0.41)× 10(-5)cm/s (n=4), an increase of nearly 300%. A z-test verified that a significant change in the permeability rate (p<0.05) had occurred after 4 days of 4°C storage. Histology was used to quantify changes in tissue pore area. The increase in average pore area paralleled the increase in permeability rate over 10 days. CONCLUSIONS: These results suggest that (1) the structural integrity of aortic tissue at 4°C is retained for at least the first three days after resection and (2) OCT is a powerful technology well suited for evaluating tissue structural integrity over time. GENERAL SIGNIFICANCE: Functional OCT imaging provides for a noninvasive and quantitative technique in determining the structural integrity of aortic tissue stored at 4°C. This modality may be used for assessing the efficacy of other preservation techniques.
Authors: J Pirenne; F Van Gelder; W Coosemans; R Aerts; B Gunson; T Koshiba; I Fourneau; D Mirza; W Van Steenbergen; J Fevery; F Nevens; P McMaster Journal: Liver Transpl Date: 2001-06 Impact factor: 5.799
Authors: Piotr Wilczek; Gach Paulina; Jendryczko Karolina; Marcisz Martyna; Wilczek Grazyna; Major Roman; Mzyk Aldona; Sypien Anna; Samotus Aneta Journal: J Mater Sci Mater Med Date: 2018-07-03 Impact factor: 3.896