| Literature DB >> 21307206 |
César G Albariño1, Brian H Bird, Ayan K Chakrabarti, Kimberly A Dodd, Bobbie Rae Erickson, Stuart T Nichol.
Abstract
Lassa virus (LASV), is a significant cause of severe, often fatal, hemorrhagic fever in humans throughout western Africa, with an estimated 100,000 infections each year. No vaccines are commercially available. We report the development of an efficient reverse genetics system to rescue recombinant LASV and to investigate the contributions of the long 5' and 3' noncoding regions (NCRs) of the S genomic segment to in vitro growth and in vivo virulence. This work demonstrates that deletions of large portions of these NCRs confer an attenuated phenotype and are a first step toward further insights into the high virulence of LASV.Entities:
Mesh：
Substances：
Year: 2011 PMID： 21307206 PMCID： PMC3126145 DOI： 10.1128/JVI.02556-10
Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103