| Literature DB >> 21306482 |
Miki Kikuchi1, Ryuhei Hayashi, Sachiko Kanakubo, Ayumi Ogasawara, Masayuki Yamato, Noriko Osumi, Kohji Nishida.
Abstract
The purpose of this study was to characterize neural crest-derived cells within the adult murine iris. The iris was isolated from P0-Cre/Floxed-EGFP transgenic (TG) mice. The isolated iris cells formed EGFP-positive spheres on non-adhesive culture plates. Immunostaining showed that these EGFP-positive spheres expressed neural crest markers including Sox10 and p75NTR, and these cells showing in vitro sphere-forming ability were originally resided in the iris stroma (IS), in vivo. Real-time RT-PCR showed that the EGFP-positive spheres expressed significantly higher levels of the neural crest markers than EGFP-negative spheres and bone marrow-derived mesenchymal stem cells. Furthermore, the iris stromal sphere had capability to differentiate into various cell lineages including smooth muscle and cartilage. These data indicate that neural crest-derived multipotent cells can be isolated from the murine IS and expanded in sphere culture.Entities:
Mesh：
Substances：
Year: 2011 PMID： 21306482 DOI： 10.1111/j.1365-2443.2011.01485.x
Source DB: PubMed Journal: Genes Cells ISSN： 1356-9597 Impact factor: 1.891