OBJECTIVES: Immediate tooth replantation is the choice of treatment in tooth avulsion. When it is impossible to replant immediately an appropriate medium must be used to maintain periodontal ligament (PDL) cell viability. The purpose of this study was to investigate the effect of standard Oral Rehydration Solution (ORS) as a new storage medium for the preservation of PDL cell viability. METHODS: 75 young adult premolar teeth without periodontal disease were used (25 teeth for each medium). Teeth were placed in the experimental media including standard ORS, HBSS and tap water for intended incubation periods. After that, PDL cells were separated and cell viability was determined by Trypan blue staining. The results were analysed by one way ANOVA. RESULTS: There was no significant difference between HBSS and Standard ORS in supporting the maintenance of PDL cell viability. A significant difference between the effect of Standard ORS and water and HBSS and water was observed in maintaining PDL cell viability. CONCLUSIONS: Standard ORS is a suitable transport medium to support the maintenance of PDL cell viability. Regarding its inexpensiveness and availability, standard ORS has preference over some other approved transport media like HBSS.
OBJECTIVES: Immediate tooth replantation is the choice of treatment in tooth avulsion. When it is impossible to replant immediately an appropriate medium must be used to maintain periodontal ligament (PDL) cell viability. The purpose of this study was to investigate the effect of standard Oral Rehydration Solution (ORS) as a new storage medium for the preservation of PDL cell viability. METHODS: 75 young adult premolar teeth without periodontal disease were used (25 teeth for each medium). Teeth were placed in the experimental media including standard ORS, HBSS and tapwater for intended incubation periods. After that, PDL cells were separated and cell viability was determined by Trypan blue staining. The results were analysed by one way ANOVA. RESULTS: There was no significant difference between HBSS and Standard ORS in supporting the maintenance of PDL cell viability. A significant difference between the effect of Standard ORS and water and HBSS and water was observed in maintaining PDL cell viability. CONCLUSIONS: Standard ORS is a suitable transport medium to support the maintenance of PDL cell viability. Regarding its inexpensiveness and availability, standard ORS has preference over some other approved transport media like HBSS.