OBJECTIVE: Stable animal models for refractory peripheral arterial disease are not established. A standardized animal model of hind-limb ischemia is required upon searching effective treatment for this condition. The aim of the study is to verify previously used hind-limb ischemia models to find a standard method. METHODS: Using Balb/ca mice six various methods of inducing hind-limb ischemia were applied and two weeks after operation degree of ischemic damage were examined. Six methods include V group, A group, AV group, A-strip group, AV-strip group and Prox-A group (refer the text). RESULTS: Degree of ischemia was evaluated macroscopically by judging toes, foot, knee, and total hind-limb necrosis. We found that severity of damage was markedly different among different methods. Furthermore the severity of necrosis was not uniform even in the same method group. CONCLUSIONS: The A-strip group in which the femoral artery from the bifurcation of the deep femoral artery to the saphenous artery was stripped appears to be suitable as a stable severe ischemia model. The A group in which the femoral artery were cut just below the bifurcation of the deep femoral artery appears to be suitable as a chronic mild ischemia model.
OBJECTIVE: Stable animal models for refractory peripheral arterial disease are not established. A standardized animal model of hind-limb ischemia is required upon searching effective treatment for this condition. The aim of the study is to verify previously used hind-limb ischemia models to find a standard method. METHODS: Using Balb/ca mice six various methods of inducing hind-limb ischemia were applied and two weeks after operation degree of ischemic damage were examined. Six methods include V group, A group, AV group, A-strip group, AV-strip group and Prox-A group (refer the text). RESULTS: Degree of ischemia was evaluated macroscopically by judging toes, foot, knee, and total hind-limb necrosis. We found that severity of damage was markedly different among different methods. Furthermore the severity of necrosis was not uniform even in the same method group. CONCLUSIONS: The A-strip group in which the femoral artery from the bifurcation of the deep femoral artery to the saphenous artery was stripped appears to be suitable as a stable severe ischemia model. The A group in which the femoral artery were cut just below the bifurcation of the deep femoral artery appears to be suitable as a chronic mild ischemia model.
Authors: Phuc Van Pham; Ngoc Bich Vu; Hoa Trong Nguyen; Thuy Thi-Thanh Dao; Ha Thi-Ngan Le; Lan Thi Phi; Oanh Thi-Kieu Nguyen; Ngoc Kim Phan Journal: In Vitro Cell Dev Biol Anim Date: 2017-04-19 Impact factor: 2.416
Authors: Robert A Brenes; Caroline C Jadlowiec; Mackenzie Bear; Peter Hashim; Clinton D Protack; Xin Li; Wei Lv; Michael J Collins; Alan Dardik Journal: J Vasc Surg Date: 2012-07-24 Impact factor: 4.268
Authors: Husain A Al-Mubarak; Talal M Alamri; Saif A Aljabab; Mohammad Atteya; Adrian Quan; Hwee Teoh; Praphulla C Shukla; Subodh Verma; Abdullah Aldahmash; Badr Aljabri; Claudio Napoli; Mohammed Al-Omran Journal: Vasc Cell Date: 2011-06-14