Literature DB >> 21300020

Isolation of high-quality RNA from various tissues of Jatropha curcas for downstream applications.

G Raja Krishna Kumar1, Nalini Eswaran, T Sudhakar Johnson.   

Abstract

A method for isolating transcriptionally active RNA for downstream applications from diverse tissues of Jatropha curcas, a plant rich in latex, lipids, waxes, polysaccharide, polyphenols, and secondary metabolites, is described. The described method uses alkaline borate buffer during tissue homogenization to negate the formation of viscous gel observed in guanidium-salt-containing methods. By this method, quality RNA was extracted from leaf, immature inflorescence, endosperm, and root tissues with yields ranging from 1.80 to 7.80mg/100mg fresh weight (FW). The total RNA obtained was found to be suitable for poly(A)(+)RNA purification, complementary DNA (cDNA) synthesis, cloning of full-length cDNA, and cDNA library construction.
Copyright © 2011 Elsevier Inc. All rights reserved.

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Year:  2011        PMID： 21300020     DOI： 10.1016/j.ab.2011.01.046

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  9 in total

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Review 3.  Molecular approaches to improvement of Jatropha curcas Linn. as a sustainable energy crop.

Authors:  T Sudhakar Johnson; Nalini Eswaran; M Sujatha
Journal:  Plant Cell Rep       Date:  2011-05-17       Impact factor: 4.570

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9.  Global analysis of transcriptome responses and gene expression profiles to cold stress of Jatropha curcas L.

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  9 in total

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