A Ostyn1, F Guillier, A-L Prufer, I Papinaud, S Messio, S Krys, B Lombard, J-A Hennekinne. 1. French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Maisons-Alfort Laboratory for Food Safety, Unit Characterization of Toxins, National and European Union Reference Laboratory for Coagulase Positive Staphylococci Including Staphylococcus aureus, Maisons-Alfort Cedex, France.
Abstract
AIM: To determine the performance of the Ridascreen® SET Total kit, after sample extraction and concentration by dialysis, with regard to its use in official controls for staphylococcal enterotoxins under European Regulation (EC) No. 2073/2005 modified. This study was conducted on naturally contaminated cheese samples and compared with the results of the previously validated Vidas® SET2 kit. METHODS AND RESULTS: The effectiveness of the Ridascreen® SET Total kit on naturally contaminated cheeses was compared to that of the Vidas® SET2 kit by applying the EN ISO 16140 standard. Sensitivity and specificity were also compared using spiked buffer solutions and cheese samples with SEA to SEE toxins. CONCLUSIONS: This study showed that the Ridascreen® SET Total kit is as effective as the Vidas® SET2 kit. SIGNIFICANCE AND IMPACT OF THE STUDY: The Ridascreen® SET Total kit was found to specifically detect SEA to SEE in cheeses. The Ridascreen® SET Total can therefore be used to check the staphylococcal enterotoxin content and ensure consumer protection.
AIM: To determine the performance of the Ridascreen® SET Total kit, after sample extraction and concentration by dialysis, with regard to its use in official controls for staphylococcal enterotoxins under European Regulation (EC) No. 2073/2005 modified. This study was conducted on naturally contaminated cheese samples and compared with the results of the previously validated Vidas® SET2 kit. METHODS AND RESULTS: The effectiveness of the Ridascreen® SET Total kit on naturally contaminated cheeses was compared to that of the Vidas® SET2 kit by applying the EN ISO 16140 standard. Sensitivity and specificity were also compared using spiked buffer solutions and cheese samples with SEA to SEE toxins. CONCLUSIONS: This study showed that the Ridascreen® SET Total kit is as effective as the Vidas® SET2 kit. SIGNIFICANCE AND IMPACT OF THE STUDY: The Ridascreen® SET Total kit was found to specifically detect SEA to SEE in cheeses. The Ridascreen® SET Total can therefore be used to check the staphylococcal enterotoxin content and ensure consumer protection.