| Literature DB >> 21298668 |
An-Bang Wang1, Chia-Wei Cheng, I-Chun Lin, Fei-Yau Lu, Huai-Jen Tsai, Chiu-Chun Lin, Chun-Hui Yang, Po-Ting Pan, Chen-Chi Kuan, Yen-Chih Chen, Yi-Wei Lin, Chih-Ning Chang, Yi-Hung Wu, Tetuko Kurniawan, Chii-Wann Lin, Andrew M Wo, Lin-Chi Chen.
Abstract
In the conventional bench-top approach, the DNA recombination process is time- and effort-consuming due to laborious procedures lasting from several hours to a day. A novel DNA selection and direct extraction process has been proposed, integrated and tested on chip. The integrative microfluidic chip can perform the whole procedure of DNA recombination, including DNA digestion, gel electrophoresis, DNA extraction and insert-vector ligation within 1 h. In this high-throughput design, the manual gel cutting was replaced by an automatic processing system that performed high-quality and high-recovery efficiency in DNA extraction process. With no need of gel-dissolving reagents and manipulation, the application of selection and direct extraction process could significantly eliminate the risks from UV and EtBr and also facilitate DNA recombination. Reliable output with high success rate of cloning has been achieved with a significant reduction in operational hazards, required materials, efforts and time.Entities:
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Year: 2010 PMID： 21298668 DOI： 10.1002/elps.201000449
Source DB: PubMed Journal: Electrophoresis ISSN： 0173-0835 Impact factor: 3.535