PURPOSE: To implement a bioinspired methodology using superhydrophobic surfaces suitable for producing smart hydrogel beads in which the bioactive substance is introduced in the particles during their formation. METHODS: Several superhydrophobic surfaces, including polystyrene, aluminum and copper, were prepared. Polymeric solutions composed by photo-crosslinked dextran-methacrylated and thermal responsive poly(N-isopropylacrylamide) mixed with a protein (insulin or albumin) were dropped on the superhydrophobic surfaces, and the obtained millimetric spheres were hardened in a dry environment under UV light. RESULTS: Spherical and non-sticky hydrogels particles were formed in few minutes on the superhydrophobic surfaces. The proteins included in the liquid formulation were homogeneously distributed in the particle network. The particles exhibited temperature-sensitive swelling, porosity and protein release rate, with the responsiveness tunable by the dextran-MA/PNIPAAm weight ratio. CONCLUSIONS: The proposed method permitted the preparation of smart hydrogel particles in one step with almost 100% encapsulation yield. The temperature-sensitive release profiles suggest that the obtained spherical-shaped biomaterials are suitable as protein carriers. These stimuli-responsive beads could have potential to be used in pharmaceutical or other biomedical applications, including tissue engineering and regenerative medicine.
PURPOSE: To implement a bioinspired methodology using superhydrophobic surfaces suitable for producing smart hydrogel beads in which the bioactive substance is introduced in the particles during their formation. METHODS: Several superhydrophobic surfaces, including polystyrene, aluminum and copper, were prepared. Polymeric solutions composed by photo-crosslinked dextran-methacrylated and thermal responsive poly(N-isopropylacrylamide) mixed with a protein (insulin or albumin) were dropped on the superhydrophobic surfaces, and the obtained millimetric spheres were hardened in a dry environment under UV light. RESULTS: Spherical and non-sticky hydrogels particles were formed in few minutes on the superhydrophobic surfaces. The proteins included in the liquid formulation were homogeneously distributed in the particle network. The particles exhibited temperature-sensitive swelling, porosity and protein release rate, with the responsiveness tunable by the dextran-MA/PNIPAAm weight ratio. CONCLUSIONS: The proposed method permitted the preparation of smart hydrogel particles in one step with almost 100% encapsulation yield. The temperature-sensitive release profiles suggest that the obtained spherical-shaped biomaterials are suitable as protein carriers. These stimuli-responsive beads could have potential to be used in pharmaceutical or other biomedical applications, including tissue engineering and regenerative medicine.
Authors: Carmen Alvarez-Lorenzo; Angel Concheiro; Alexander S Dubovik; Natalia V Grinberg; Tatiana V Burova; Valerij Ya Grinberg Journal: J Control Release Date: 2005-02-16 Impact factor: 9.776
Authors: Adriano V Reis; André R Fajardo; Ivania T A Schuquel; Marcos R Guilherme; Gentil José Vidotti; Adley F Rubira; Edvani C Muniz Journal: J Org Chem Date: 2009-05-15 Impact factor: 4.354
Authors: Fernanda Araiza-Verduzco; Eustolia Rodríguez-Velázquez; Harold Cruz; Ignacio A Rivero; Delvis R Acosta-Martínez; Georgina Pina-Luis; Manuel Alatorre-Meda Journal: Materials (Basel) Date: 2020-01-22 Impact factor: 3.623