OBJECTIVE: To determine the effect of various dietary components on the intestinal uptake of lutein in aged rats. METHODS: This study determined the time-course (2, 4, 6, 8 h) plasma and tissue responses of a pharmacologic dose of lutein (200 μM) solubilized in mixed micelles with fat (3%, soybean oil), phosphatidylcholine (PC; 3 mM), lysophosphatidylcholine (lysoPC; 3 mM), dietary fiber (pectin, 1.25%), β-carotene (200 μM), or micelles with no dietary components (control) in aged rats with lutein deficiency. RESULTS: No lutein was detected in the plasma of rats at 0 h indicating the deficiency. After gavages of lutein, the mean percent area under the curve (picomoles per milliliter per 8 h) of plasma lutein in the fat (91.4), PC (218.0), and lysoPC (94.1) groups were higher (P > 0.05), whereas its level in the dietary fiber and β-carotene groups was lower than the control group. The liver and eye lutein levels of the PC (95.4, 38.67%) and fat (18.2, 143%) groups were significantly higher, whereas the lysoPC (9.6, 27.2%), β-carotene (19.2, 35.4%), and dietary fiber (3.1, 88.4%) groups were lower than the control group. CONCLUSION: Results indicate that soybean oil and soy phospholipids greatly sway lutein absorption in aged rats with lutein deficiency. The results also suggest that ingestion of lutein with pectin and β-carotene suppresses lutein absorption. Hence, to improve the absorption of lutein in older adults with macular pigment deficiency, foods with sufficient fat with low dietary fiber and β-carotene may be suggested.
OBJECTIVE: To determine the effect of various dietary components on the intestinal uptake of lutein in aged rats. METHODS: This study determined the time-course (2, 4, 6, 8 h) plasma and tissue responses of a pharmacologic dose of lutein (200 μM) solubilized in mixed micelles with fat (3%, soybean oil), phosphatidylcholine (PC; 3 mM), lysophosphatidylcholine (lysoPC; 3 mM), dietary fiber (pectin, 1.25%), β-carotene (200 μM), or micelles with no dietary components (control) in aged rats with lutein deficiency. RESULTS: No lutein was detected in the plasma of rats at 0 h indicating the deficiency. After gavages of lutein, the mean percent area under the curve (picomoles per milliliter per 8 h) of plasma lutein in the fat (91.4), PC (218.0), and lysoPC (94.1) groups were higher (P > 0.05), whereas its level in the dietary fiber and β-carotene groups was lower than the control group. The liver and eye lutein levels of the PC (95.4, 38.67%) and fat (18.2, 143%) groups were significantly higher, whereas the lysoPC (9.6, 27.2%), β-carotene (19.2, 35.4%), and dietary fiber (3.1, 88.4%) groups were lower than the control group. CONCLUSION: Results indicate that soybean oil and soy phospholipids greatly sway lutein absorption in aged rats with lutein deficiency. The results also suggest that ingestion of lutein with pectin and β-carotene suppresses lutein absorption. Hence, to improve the absorption of lutein in older adults with macular pigment deficiency, foods with sufficient fat with low dietary fiber and β-carotene may be suggested.
Authors: G Sowmya Shree; K Yogendra Prasad; H S Arpitha; U R Deepika; K Nawneet Kumar; Priya Mondal; P Ganesan Journal: Mol Cell Biochem Date: 2017-05-26 Impact factor: 3.396
Authors: Joana Díaz-Gómez; Jose A Moreno; Eduardo Angulo; Gerhard Sandmann; Changfu Zhu; Teresa Capell; Carmina Nogareda Journal: Transgenic Res Date: 2017-06-23 Impact factor: 2.788
Authors: Binxing Li; Preejith P Vachali; Zhengqing Shen; Aruna Gorusupudi; Kelly Nelson; Brian M Besch; Alexis Bartschi; Simone Longo; Ty Mattinson; Saeed Shihab; Nikolay E Polyakov; Lyubov P Suntsova; Alexander V Dushkin; Paul S Bernstein Journal: Exp Eye Res Date: 2017-03-09 Impact factor: 3.467