BACKGROUND: Recently a somatic point mutation in the FOXL2 gene has been characterized in ovarian adult type of granulosa cell tumor (ATGCT) (94.6%), thecomas (12.5%), but not in juvenile type of ovarian granulosa cell tumor, other ovarian sex cord tumors and ovarian surface epithelial neoplasms. Whether this mutation is present in testicular ATGCT or incompletely differentiated sex cord stromal tumor (ISCST) is not known. DESIGN: Four ATGCTs, 4 ISCST were immunohistochemically investigated with anti-FOXL2 and 3 ovarian ATGCTs were used as positive control. RESULTS: Weak-to-moderate immunoreactivity was found in all tested testicular and ovarian tumors. PCR and direct sequencing were used for detection of c.402C>G of the FOXL2 gene. No mutation was found in any of the testicular ATGCTs or ISCSTs whereas all ovarian tumors showed the c.402C>G point mutation of the FOXL2 gene. CONCLUSIONS: On the basis of this small series of these rare testicular neoplasms, it seems that the c.402C>G mutation of the FOXL2 gene frequently found in adult type of ovarian GCT does not play any significant role in the development of ATGCT and ISCST.
BACKGROUND: Recently a somatic point mutation in the FOXL2 gene has been characterized in ovarian adult type of granulosa cell tumor (ATGCT) (94.6%), thecomas (12.5%), but not in juvenile type of ovarian granulosa cell tumor, other ovarian sex cord tumors and ovarian surface epithelial neoplasms. Whether this mutation is present in testicular ATGCT or incompletely differentiated sex cord stromal tumor (ISCST) is not known. DESIGN: Four ATGCTs, 4 ISCST were immunohistochemically investigated with anti-FOXL2 and 3 ovarian ATGCTs were used as positive control. RESULTS: Weak-to-moderate immunoreactivity was found in all tested testicular and ovarian tumors. PCR and direct sequencing were used for detection of c.402C>G of the FOXL2 gene. No mutation was found in any of the testicular ATGCTs or ISCSTs whereas all ovarian tumors showed the c.402C>G point mutation of the FOXL2 gene. CONCLUSIONS: On the basis of this small series of these rare testicular neoplasms, it seems that the c.402C>G mutation of the FOXL2 gene frequently found in adult type of ovarian GCT does not play any significant role in the development of ATGCT and ISCST.
Authors: Anthony N Karnezis; Kathleen R Cho; C Blake Gilks; Celeste Leigh Pearce; David G Huntsman Journal: Nat Rev Cancer Date: 2016-11-25 Impact factor: 60.716
Authors: Melissa K McConechy; Anniina Färkkilä; Hugo M Horlings; Aline Talhouk; Leila Unkila-Kallio; Hannah S van Meurs; Winnie Yang; Nirit Rozenberg; Noora Andersson; Katharina Zaby; Saara Bryk; Ralf Bützow; Johannes B G Halfwerk; Gerrit K J Hooijer; Marc J van de Vijver; Marrije R Buist; Gemma G Kenter; Sara Y Brucker; Bernhard Krämer; Annette Staebler; Maaike C G Bleeker; Markku Heikinheimo; Stefan Kommoss; C Blake Gilks; Mikko Anttonen; David G Huntsman Journal: J Natl Cancer Inst Date: 2016-06-13 Impact factor: 13.506
Authors: Sigrid Marie Kraggerud; Christina E Hoei-Hansen; Sharmini Alagaratnam; Rolf I Skotheim; Vera M Abeler; Ewa Rajpert-De Meyts; Ragnhild A Lothe Journal: Endocr Rev Date: 2013-04-10 Impact factor: 19.871