OBJECTIVES: This study aimed to investigate new biomarkers of obesity particularly in relation with inflammation-associated proteins using protein differential display techniques. DESIGN AND METHODS: Comparison of protein expression in plasma between non-obese (n=109, body mass index, BMI<25kg/m(2)) and obese (n=32, BMI≥25kg/m(2)) groups was carried out using two-dimensional gel electrophoresis (2-DE) analysis. ELISA was also performed for validation. RESULTS: Among six differentially expressed protein spots, ceruloplasmin (Cp) and fibrinogen were over-expressed in obese group. Plasma Cp levels were significantly higher in obese group than non-obese group (34.0 ± 8.6 vs. 41.3 ± 12.7mg/dL, p<0.001) and positively correlated with age (r=0.253, p<0.005), BMI (r=0.265, p<0.001) and hsCRP (r=0.385, p<0.001). In stepwise multiple linear regression analysis, plasma Cp along with hsCRP were found predictors for obesity (adjusted β-coefficient=0.266, p<0.01). CONCLUSION: Elevated plasma Cp levels were significantly associated with obesity, which may be suggested to be a marker of obesity.
OBJECTIVES: This study aimed to investigate new biomarkers of obesity particularly in relation with inflammation-associated proteins using protein differential display techniques. DESIGN AND METHODS: Comparison of protein expression in plasma between non-obese (n=109, body mass index, BMI<25kg/m(2)) and obese (n=32, BMI≥25kg/m(2)) groups was carried out using two-dimensional gel electrophoresis (2-DE) analysis. ELISA was also performed for validation. RESULTS: Among six differentially expressed protein spots, ceruloplasmin (Cp) and fibrinogen were over-expressed in obese group. Plasma Cp levels were significantly higher in obese group than non-obese group (34.0 ± 8.6 vs. 41.3 ± 12.7mg/dL, p<0.001) and positively correlated with age (r=0.253, p<0.005), BMI (r=0.265, p<0.001) and hsCRP (r=0.385, p<0.001). In stepwise multiple linear regression analysis, plasma Cp along with hsCRP were found predictors for obesity (adjusted β-coefficient=0.266, p<0.01). CONCLUSION: Elevated plasma Cp levels were significantly associated with obesity, which may be suggested to be a marker of obesity.
Authors: N Tajik; A Golpaie; S A Keshavarz; M Djalali; M Sehat; F Masoudkabir; Z Ahmadivand; F Fatehi; M Zare; T Yazdani Journal: J Endocrinol Invest Date: 2011-07-27 Impact factor: 4.256
Authors: Helen Hermana M Hermsdorff; Ana Carolina P Volp; Blanca Puchau; Kiriaque B F Barbosa; M Angeles Zulet; Josefina Bressan; J Alfredo Martínez Journal: Inflamm Res Date: 2012-01-19 Impact factor: 4.575
Authors: Haojun Yang; Chin-Nung Liu; Risa M Wolf; Martina Ralle; Som Dev; Hannah Pierson; Frederic Askin; Kimberley E Steele; Thomas H Magnuson; Michael A Schweitzer; G William Wong; Svetlana Lutsenko Journal: Metallomics Date: 2019-06-28 Impact factor: 4.526
Authors: Razvan T Dadu; Rhiannon Dodge; Vijay Nambi; Salim S Virani; Ron C Hoogeveen; Nicholas L Smith; Fengju Chen; James S Pankow; Cameron Guild; W H Wilson Tang; Eric Boerwinkle; Stanley L Hazen; Christie M Ballantyne Journal: Circ Heart Fail Date: 2013-07-16 Impact factor: 8.790
Authors: Erik Arner; Alistair R R Forrest; Anna Ehrlund; Niklas Mejhert; Masayoshi Itoh; Hideya Kawaji; Timo Lassmann; Jurga Laurencikiene; Mikael Rydén; Peter Arner Journal: PLoS One Date: 2014-03-27 Impact factor: 3.240