Determination of affinities of murine and chimeric anti alpha/beta-T-cell receptor antibodies by flow cytometry.

In this report we describe the use of a calibrated flow cytometer to quantify the expression of the human alpha/beta-T-cell-receptor on peripheral blood lymphocytes of healthy human blood donors and on the T-cell line HPB-ALL. To calculate the maximum of antibodies which are needed to obtain antigen saturation per cell and to determine the affinity of murine and chimeric anti-TCR antibodies, we applied Scatchard-plot and double reciprocal Klotz-plot analyses.