Literature DB >> 21288739

Bi-allelic gene targeting in mouse embryonic stem cells.

Peri H Tate1, William C Skarnes.   

Abstract

The EUCOMM and KOMP programs have generated targeted conditional alleles in mouse embryonic stem cells for nearly 10,000 genes. The availability of these stem cell resources will greatly accelerate the functional analysis of genes in mice and in cultured cells. We present a method for conditional ablation of genes in ES cells using vectors and targeted clones from the EUCOMM and KOMP conditional resources. Inducible homozygous cells described here provide a precisely controlled experimental system to study gene function in a model cell.
Copyright © 2011 Elsevier Inc. All rights reserved.

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Year:  2011        PMID： 21288739      PMCID： PMC3572411          DOI： 10.1016/j.ymeth.2010.12.025

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  35 in total

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Authors:  M J Evans; M H Kaufman
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5.  Ligand-activated site-specific recombination in mice.

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Journal:  Proc Natl Acad Sci U S A       Date:  1996-10-01       Impact factor: 11.205

6.  Embryonic stem cell lines derived from human blastocysts.

Authors:  J A Thomson; J Itskovitz-Eldor; S S Shapiro; M A Waknitz; J J Swiergiel; V S Marshall; J M Jones
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7.  A new logic for DNA engineering using recombination in Escherichia coli.

Authors:  Y Zhang; F Buchholz; J P Muyrers; A F Stewart
Journal:  Nat Genet       Date:  1998-10       Impact factor: 38.330

8.  The knockout mouse project.

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Journal:  Nat Genet       Date:  2004-09       Impact factor: 38.330

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Journal:  Nat Genet       Date:  2004-09       Impact factor: 38.330
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  12 in total

Review 1.  Utility of genetically modified mice for understanding the neurobiology of substance use disorders.

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Journal:  Hum Genet       Date:  2011-12-22       Impact factor: 4.132

2.  Use of two gRNAs for CRISPR/Cas9 improves bi-allelic homologous recombination efficiency in mouse embryonic stem cells.

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Journal:  Genesis       Date:  2018-05-11       Impact factor: 2.487

3.  Lack of evidence for participation of TMEM150C in sensory mechanotransduction.

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4.  Permissive and restricted virus infection of murine embryonic stem cells.

Authors:  Rachael Wash; Sabrina Calabressi; Stephanie Franz; Samantha J Griffiths; David Goulding; E-Pien Tan; Helen Wise; Paul Digard; Jürgen Haas; Stacey Efstathiou; Paul Kellam
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5.  Isolation of homozygous mutant mouse embryonic stem cells using a dual selection system.

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6.  One-step generation of conditional and reversible gene knockouts.

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8.  Loss of IL-10 signaling in macrophages limits bacterial killing driven by prostaglandin E2.

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9.  Conditional-ready mouse embryonic stem cell derived macrophages enable the study of essential genes in macrophage function.

Authors:  A T Y Yeung; C Hale; J Xia; P H Tate; D Goulding; J A Keane; S Mukhopadhyay; L Forrester; O Billker; W C Skarnes; R E W Hancock; G Dougan
Journal:  Sci Rep       Date:  2015-03-10       Impact factor: 4.379

10.  An efficient method for generation of bi-allelic null mutant mouse embryonic stem cells and its application for investigating epigenetic modifiers.

Authors:  Cynthia L Fisher; Hendrik Marks; Lily Ting-Yin Cho; Robert Andrews; Sam Wormald; Thomas Carroll; Vivek Iyer; Peri Tate; Barry Rosen; Hendrik G Stunnenberg; Amanda G Fisher; William C Skarnes
Journal:  Nucleic Acids Res       Date:  2017-12-01       Impact factor: 16.971
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