Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Developmental expression of choline acetyltransferase mRNA in Drosophila.

Literature DB >> 2128533

Developmental expression of choline acetyltransferase mRNA in Drosophila.

L A Carbini¹, V J Muñoz Maines, P M Salvaterra.

Abstract

We have measured the steady state levels of choline acetyltransferase (ChAT, EC 2.3.1.6) mRNA during different developmental stages of Drosophila melanogaster using a ChAT specific cRNA probe. ChAT mRNA was first detected approximately 6-7 h after oviposition, increased until the 1st-2nd larval instar, decreased into early pupal stages and increased again during late pupation, reaching a maximum in adults. Northern analysis showed a major RNA band with a Mr of 4.7 kilobases and Western analysis also showed a single major 75 kD protein band at all developmental stages. Our results support the hypothesis that a major point of regulation of ChAT expression may be at the transcriptional level.

Entities: Gene Species

Mesh：

Substances：

Year: 1990 PMID： 2128533 DOI： 10.1007/bf01101709

Source DB: PubMed Journal: Neurochem Res ISSN： 0364-3190 Impact factor: 3.996

23 in total

Review 1. Eukaryotic nuclear RNA polymerases.

Authors: P Chambon
Journal: Annu Rev Biochem Date: 1975 Impact factor: 23.643

2. Localization of Drosophila neurons that contain choline acetyltransferase messenger RNA: an in situ hybridization study.

Authors: R P Barber; H Sugihara; M Lee; J E Vaughn; P M Salvaterra
Journal: J Comp Neurol Date: 1989-02-22 Impact factor: 3.215

3. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

3. Abnormal chemosensory jump 6 is a positive transcriptional regulator of the cholinergic gene locus in Drosophila olfactory neurons.

Authors: Mi-Heon Lee; Paul M Salvaterra
Journal: J Neurosci Date: 2002-07-01 Impact factor: 6.167

3 in total

Developmental expression of choline acetyltransferase mRNA in Drosophila.

Review 1. Eukaryotic nuclear RNA polymerases.

2. Localization of Drosophila neurons that contain choline acetyltransferase messenger RNA: an in situ hybridization study.

3. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

4. Single RNA species injected in Xenopus oocyte directs the synthesis of active choline acetyltransferase.

5. Metabolism of biogenic amines in Drosophila nervous tissue.

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

7. The time course of development of acetylcholinesterase and choline acetyltransferase in Drosophila melanogaster.

8. Production of polyclonal antisera to choline acetyltransferase using a fusion protein produced by a cDNA clone.

9. Differentiation of Drosophila neuroblasts to form ganglion-like clusters of neurons in vitro.

10. Immunocytochemical study of a temperature-sensitive choline acetyltransferase mutant of Drosophila melanogaster.

1. Temporal and spatial expression patterns of two G-protein coupled receptors in Drosophila melanogaster.

2. Developmental regulatory elements in the 5' flanking DNA of the Drosophila choline acetyltransferase gene.

3. Abnormal chemosensory jump 6 is a positive transcriptional regulator of the cholinergic gene locus in Drosophila olfactory neurons.