| Literature DB >> 21281400 |
Hirokazu Okayama1, Kensuke Kumamoto, Katsuharu Saitou, Suguru Hayase, Yasuhide Kofunato, Yu Sato, Kotaro Miyamoto, Izumi Nakamura, Shinji Ohki, Yoshihisa Koyama, Yoshimasa Ishii, Seiichi Takenoshita.
Abstract
The aim of this study was to clarify the clinical implications of a unique carbohydrate determinant, MECA-79, in gastric cancer specimens and cells. Immunohistochemical analysis showed that 62 of 225 (27.6%) cases were defined as positive for MECA-79. MECA-79 expression was correlated with depth of invasion, venous invasion, TNM stage, and distant metastasis. In survival analyses, patients with MECA-79 expression had worse prognosis by the log-rank test. Multivariate analysis of the Cox proportional hazard model showed that MECA-79 expression was an independent factor of a worse cancer-specific survival. Among 11 gastric cancer cells, MECA-79 was observed in only MKN7 cells, which also expressed GlcNAc6ST-2 transcript. A knockdown of GlcNAc6ST-2 in MKN7 cells showed a markedly reduced expression of MECA-79, suggesting that GlcNAc-sulfation of MECA-79 is mainly synthesized by GlcNAc6ST-2. Furthermore, real-time RT-PCR analysis revealed that GlcNAc6ST-2 was significantly increased in cancer tissues compared with paired normal mucosa. In conclusion, the expression of MECA-79 could be a useful marker for the prognosis of gastric cancer. Our results might also provide novel perspectives on the biology of MECA-79 and GlcNAc6ST-2 in cancer progression and metastasis.Entities:
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Year: 2011 PMID： 21281400 DOI： 10.1111/j.1349-7006.2011.01895.x
Source DB: PubMed Journal: Cancer Sci ISSN： 1347-9032 Impact factor: 6.716