AIMS: Legionella isolation from environmental samples is often difficult because of the presence of heterotrophic-associated bacteria that frequently overgrow when using standard culture (ISO 11731, 1998; NF T90-431, 2003) methods. To improve Legionella pneumophila recovery from complex water samples (water from cooling towers, biofilms), we evaluated an immunomagnetic separation (IMS) assay using a monoclonal antibody raised against the lipopolysaccharide of Leg. pneumophila sg1 in combination with culture. METHODS AND RESULTS: This study was conducted on 51 environmental specimens. The comparison between IMS-culture and standard culture (ISO 11731, 1998; NF T90-431, 2003) methods was made using ISO 17994, 2004 criteria for establishing equivalence between microbiological methods based on the upper and lower (XH and XL) values of the relative difference (95% confidence limit) and D as maximum acceptable deviation (value of the confidence limit). CONCLUSIONS: We found that the average performance of IMS culture was higher than the reference method.
AIMS: Legionella isolation from environmental samples is often difficult because of the presence of heterotrophic-associated bacteria that frequently overgrow when using standard culture (ISO 11731, 1998; NF T90-431, 2003) methods. To improve Legionella pneumophila recovery from complex water samples (water from cooling towers, biofilms), we evaluated an immunomagnetic separation (IMS) assay using a monoclonal antibody raised against the lipopolysaccharide of Leg. pneumophila sg1 in combination with culture. METHODS AND RESULTS: This study was conducted on 51 environmental specimens. The comparison between IMS-culture and standard culture (ISO 11731, 1998; NF T90-431, 2003) methods was made using ISO 17994, 2004 criteria for establishing equivalence between microbiological methods based on the upper and lower (XH and XL) values of the relative difference (95% confidence limit) and D as maximum acceptable deviation (value of the confidence limit). CONCLUSIONS: We found that the average performance of IMS culture was higher than the reference method.
Authors: Hong Wang; Emilie Bédard; Michèle Prévost; Anne K Camper; Vincent R Hill; Amy Pruden Journal: Water Res Date: 2017-03-25 Impact factor: 11.236
Authors: Ali Mohammadi; Stephen T Chambers; Amy Scott-Thomas; John G Lewis; Trevor Anderson; Ros Podmore; Jonathan Williman; David Murdoch; Sandy Slow Journal: J Clin Microbiol Date: 2020-10-21 Impact factor: 5.948
Authors: Álvaro Díaz-Flores; Juan Carlos Montero; Francisco Javier Castro; Eva María Alejandres; Carmen Bayón; Inmaculada Solís; Roberto Fernández-Lafuente; Guillermo Rodríguez Journal: BMC Microbiol Date: 2015-04-29 Impact factor: 3.605
Authors: Barbara Schrammel; Markus Petzold; Sílvia Cervero-Aragó; Regina Sommer; Christian Lück; Alexander Kirschner Journal: BMC Microbiol Date: 2018-07-17 Impact factor: 3.605