OBJECTIVE: Human thymic stromal lymphopoietin (TSLP), an epithelial cell-derived cytokine, promotes inflammatory T helper type 2 cell (Th2) differentiation of naive CD4(+) T cells. TSLP is highly produced in keratinocytes of patients with atopic dermatitis and bronchial epithelia of patients with asthma and was thought to be a master switch for allergic inflammation. We sought to examine the effect of TSLP in human monocytes/macrophages. METHODS: The effect of TSLP on the expression of cell surface antigens (CD11c, CD16, CD54, CD80, CD86, and HLA-DR) in peripheral blood CD14(+) monocytes/macrophages was examined. RESULTS: TSLP enhanced the expression of CD80 in peripheral blood CD14(+) monocytes/macrophages but not that of other cell surface antigens. It was associated with an increased percentage of CD14(dim/-), CD80(+), CD11c(+), and HLA-DR(+) cells, which was consistent with the increased differentiation of myeloid dendritic cells. CONCLUSIONS: TSLP induces CD80 expression in human peripheral blood CD14(+) monocytes/macrophages; this indicates monocyte/macrophage activation. This may be associated with their differentiation into myeloid dendritic cells.
OBJECTIVE:Humanthymic stromal lymphopoietin (TSLP), an epithelial cell-derived cytokine, promotes inflammatory T helper type 2 cell (Th2) differentiation of naive CD4(+) T cells. TSLP is highly produced in keratinocytes of patients with atopic dermatitis and bronchial epithelia of patients with asthma and was thought to be a master switch for allergic inflammation. We sought to examine the effect of TSLP in human monocytes/macrophages. METHODS: The effect of TSLP on the expression of cell surface antigens (CD11c, CD16, CD54, CD80, CD86, and HLA-DR) in peripheral blood CD14(+) monocytes/macrophages was examined. RESULTS:TSLP enhanced the expression of CD80 in peripheral blood CD14(+) monocytes/macrophages but not that of other cell surface antigens. It was associated with an increased percentage of CD14(dim/-), CD80(+), CD11c(+), and HLA-DR(+) cells, which was consistent with the increased differentiation of myeloid dendritic cells. CONCLUSIONS:TSLP induces CD80 expression in human peripheral blood CD14(+) monocytes/macrophages; this indicates monocyte/macrophage activation. This may be associated with their differentiation into myeloid dendritic cells.
Authors: P A Reche; V Soumelis; D M Gorman; T Clifford; M Travis; S M Zurawski; J Johnston; Y J Liu; H Spits; R de Waal Malefyt; R A Kastelein; J F Bazan Journal: J Immunol Date: 2001-07-01 Impact factor: 5.422
Authors: M Isidoro-García; I Dávila-González; M Pascual de Pedro; C Sanz-Lozano; F Lorente-Toledano Journal: Allergol Immunopathol (Madr) Date: 2007 Nov-Dec Impact factor: 1.667
Authors: L S Park; U Martin; K Garka; B Gliniak; J P Di Santo; W Muller; D A Largaespada; N G Copeland; N A Jenkins; A G Farr; S F Ziegler; P J Morrissey; R Paxton; J E Sims Journal: J Exp Med Date: 2000-09-04 Impact factor: 14.307
Authors: Ludger Klimek; Jan Hagemann; Hans-Jürgen Welkoborsky; Mandy Cuevas; Ingrid Casper; Ulrike Förster-Ruhrmann; Felix Klimek; Constantin A Hintschich; Tilman Huppertz; Christoph Bergmann; Peter-Valentin Tomazic; Sven Becker Journal: Allergol Select Date: 2022-04-29
Authors: Jason S Lehmann; Joseph J Campo; Micheline Cicéron; Christian P Raccurt; Jacques Boncy; Valery E M Beau De Rochars; Anthony P Cannella Journal: PLoS One Date: 2017-04-03 Impact factor: 3.240
Authors: J J Nieto-Fontarigo; F J Salgado; M E San-José; M J Cruz; A Casas-Fernández; M J Gómez-Conde; L Valdés-Cuadrado; M Á García-González; P Arias; M Nogueira; F J González-Barcala Journal: Sci Rep Date: 2018-03-07 Impact factor: 4.379