Literature DB >> 212742

Temperature-sensitive replication of H5ts125 adenovirus DNA in vitro.

M S Horwitz.   

Abstract

A soluble adenovirus DNA replication system has been prepared in extracts of HeLa cells infected with the temperature-sensitive mutant H5ts125. These nuclear extracts synthesize full-sized viral DNA at 30 degrees but are temperature sensitive in this function at 38 degrees. A 72,000-dalton DNA-binding protein purified from cells infected with wild-type virus complements the temperature-sensitive defect and restores viral DNA replication to 74% of normal values.

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Year:  1978        PMID： 212742      PMCID： PMC336099          DOI： 10.1073/pnas.75.9.4291

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  29 in total

1.  Amino acid metabolism in mammalian cell cultures.

Authors:  H EAGLE
Journal:  Science       Date:  1959-08-21       Impact factor: 47.728

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Subnuclear systems for synthesis of simian virus 40 DNA in vitro.

Authors:  H J Edenberg; M A Waqar; J A Huberman
Journal:  Proc Natl Acad Sci U S A       Date:  1976-12       Impact factor: 11.205

4.  Structural analysis of viral replicative intermediates isolated from adenovirus type 2-infected HeLa cell nuclei.

Authors:  C Kedinger; O Brison; F Perrin; J Wilhelm
Journal:  J Virol       Date:  1978-05       Impact factor: 5.103

5.  A map of cytoplasmic RNA transcripts from lytic adenovirus type 2, determined by electron microscopy of RNA:DNA hybrids.

Authors:  L T Chow; J M Roberts; J B Lewis; T R Broker
Journal:  Cell       Date:  1977-08       Impact factor: 41.582

6.  Adenovirus deoxyribonucleic acid replication. Isolation of a soluble replication system and analysis of the in vitro DNA product.

Authors:  T Yamashita; M Arens; M Green
Journal:  J Biol Chem       Date:  1977-11-25       Impact factor: 5.157

7.  Enzymatic properties of viral replication complexes isolated from adenovirus type 2-infected HeLa cell nuclei.

Authors:  O Brison; C Kedinger; J Wilhelm
Journal:  J Virol       Date:  1977-11       Impact factor: 5.103

8.  Replication of adenovirus type 2 DNA in vitro.

Authors:  L M Kaplan; R E Kelinman; M S Horwitz
Journal:  Proc Natl Acad Sci U S A       Date:  1977-10       Impact factor: 11.205

9.  In vitro replication of simian virus 40 DNA in a nucleoprotein complex.

Authors:  R T Su; M L DePamphilis
Journal:  Proc Natl Acad Sci U S A       Date:  1976-10       Impact factor: 11.205

10.  Purification and molecular characterization of adenovirus type 2 DNA-binding protein.

Authors:  K Sugawara; Z Gilead; M Green
Journal:  J Virol       Date:  1977-01       Impact factor: 5.103
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  32 in total

1.  Intranuclear targeting and nuclear export of the adenovirus E1B-55K protein are regulated by SUMO1 conjugation.

Authors:  Kathrin Kindsmüller; Peter Groitl; Barbara Härtl; Paola Blanchette; Joachim Hauber; Thomas Dobner
Journal:  Proc Natl Acad Sci U S A       Date:  2007-04-11       Impact factor: 11.205

2.  Localization of the adenovirus early region 1B 55-kilodalton protein during lytic infection: association with nuclear viral inclusions requires the early region 4 34-kilodalton protein.

Authors:  D A Ornelles; T Shenk
Journal:  J Virol       Date:  1991-01       Impact factor: 5.103

3.  Complementation of the temperature-sensitive defect in H5ts125 adenovirus DNA replication in vitro.

Authors:  L M Kaplan; H Ariga; J Hurwitz; M S Horwitz
Journal:  Proc Natl Acad Sci U S A       Date:  1979-11       Impact factor: 11.205

4.  Association of the adenovirus DNA-binding protein with RNA both in vitro and in vivo.

Authors:  V G Cleghon; D F Klessig
Journal:  Proc Natl Acad Sci U S A       Date:  1986-12       Impact factor: 11.205

5.  Purification and characterization of an early glycoprotein from adenovirus type 2-infected cells.

Authors:  H Persson; C Signäs; L Philipson
Journal:  J Virol       Date:  1979-03       Impact factor: 5.103

6.  Restricted changes in the adenovirus DNA-binding protein that lead to extended host range or temperature-sensitive phenotypes.

Authors:  D E Brough; S A Rice; S Sell; D F Klessig
Journal:  J Virol       Date:  1985-07       Impact factor: 5.103

7.  Identification of adenovirus 2 early genes required for induction of cellular DNA synthesis in resting hamster cells.

Authors:  M Rossini; G J Jonak; R Baserga
Journal:  J Virol       Date:  1981-06       Impact factor: 5.103

8.  Structure and organization of the gene coding for the DNA binding protein of adenovirus type 5.

Authors:  W Kruijer; F M van Schaik; J S Sussenbach
Journal:  Nucleic Acids Res       Date:  1981-09-25       Impact factor: 16.971

9.  Adeno-associated virus helper activity of adenovirus DNA binding protein.

Authors:  R A McPherson; H S Ginsberg; J A Rose
Journal:  J Virol       Date:  1982-11       Impact factor: 5.103

10.  The DNA polymerases associated with the adenovirus type 2 replication complex: effect of 2'-3'-dideoxythymidine-5'-triphosphate on viral DNA synthesis.

Authors:  M M Abboud; M S Horwitz
Journal:  Nucleic Acids Res       Date:  1979-03       Impact factor: 16.971
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