BACKGROUND: The Wnt target LGR5 has been recently identified as a murine intestinal stem cell marker. Its role during each stage of human colorectal tumorigenesis remains to be determined. PATIENTS AND METHODS: LGR5 expression was investigated by immunohistochemical analysis in 17 low-grade and 13 high-grade intraepithelial neoplasias and 30 adenocarcinomas. RESULTS: The number of LGR5-positive cells increased tumor, with clustering of the cells to form patches. An apparent difference in their distribution among the tumorigenesis stages was observed. LGR5 expression in luminal surface showed a negative association with the progressive grade of tumors, while that in lower crypt exhibited a positive association with grade. In adenocarcinomas, LGR5 expression in luminal surface was negatively associated with pStage, while it was almost invariably high in lower crypt during advanced pStage disease. CONCLUSION: These results suggest that the shifts in the distribution of LGR5-positive cells towards the lower crypt play a role in the development and progression of colorectal cancer.
BACKGROUND: The Wnt target LGR5 has been recently identified as a murine intestinal stem cell marker. Its role during each stage of human colorectal tumorigenesis remains to be determined. PATIENTS AND METHODS: LGR5 expression was investigated by immunohistochemical analysis in 17 low-grade and 13 high-grade intraepithelial neoplasias and 30 adenocarcinomas. RESULTS: The number of LGR5-positive cells increased tumor, with clustering of the cells to form patches. An apparent difference in their distribution among the tumorigenesis stages was observed. LGR5 expression in luminal surface showed a negative association with the progressive grade of tumors, while that in lower crypt exhibited a positive association with grade. In adenocarcinomas, LGR5 expression in luminal surface was negatively associated with pStage, while it was almost invariably high in lower crypt during advanced pStage disease. CONCLUSION: These results suggest that the shifts in the distribution of LGR5-positive cells towards the lower crypt play a role in the development and progression of colorectal cancer.
Authors: Kirstine Lavrsen; Sally Dabelsteen; Sergey Y Vakhrushev; Asha M R Levann; Amalie Dahl Haue; August Dylander; Ulla Mandel; Lars Hansen; Morten Frödin; Eric P Bennett; Hans H Wandall Journal: J Biol Chem Date: 2017-11-29 Impact factor: 5.157
Authors: Maria Laura Martin; Zhaoshi Zeng; Mohammad Adileh; Adrian Jacobo; Christy Li; Efsevia Vakiani; Guoqiang Hua; Lixing Zhang; Adriana Haimovitz-Friedman; Zvi Fuks; Richard Kolesnick; Philip B Paty Journal: Cell Signal Date: 2017-09-25 Impact factor: 4.315
Authors: Bo Gun Jang; Cheol Lee; Hye Sung Kim; Myung Soo Shin; Min Seok Cheon; Jae Wang Kim; Woo Ho Kim Journal: Virchows Arch Date: 2017-01-09 Impact factor: 4.064
Authors: Manal R A Al-Kharusi; Helena J M Smartt; Alexander Greenhough; Tracey J Collard; Elizabeth D Emery; Ann C Williams; Chris Paraskeva Journal: Carcinogenesis Date: 2013-01-24 Impact factor: 4.944