BACKGROUND: The molecular mechanisms of genistein's proliferative effects on breast cancer cells are largely unknown. This study aimed to examine estrogen-receptor (ER)-related signaling molecules involved in genistein-associated cell proliferation and survival (ERK1/2, p90RSK, JNK, Akt and NFκB) and to correlate these results to cell proliferation. MATERIALS AND METHODS: The effect of genistein on cell-signaling molecules was determined in T47D breast cancer cells by a Bioplex phosphoprotein detection kit. These results were confirmed by Western blotting and were correlated to cell proliferation by MTT assay. RESULTS: Low and high concentrations of genistein induced an ERK1/2-independent decrease in phosphorylated p90RSK. This effect was accompanied by decreased cell proliferation at high concentrations and an increased response at low concentrations of genistein following a 48-hour exposure. CONCLUSION: Concentration-dependent actions of genistein in T47D cells may be due to differential activation of signaling molecules.
BACKGROUND: The molecular mechanisms of genistein's proliferative effects on breast cancer cells are largely unknown. This study aimed to examine estrogen-receptor (ER)-related signaling molecules involved in genistein-associated cell proliferation and survival (ERK1/2, p90RSK, JNK, Akt and NFκB) and to correlate these results to cell proliferation. MATERIALS AND METHODS: The effect of genistein on cell-signaling molecules was determined in T47D breast cancer cells by a Bioplex phosphoprotein detection kit. These results were confirmed by Western blotting and were correlated to cell proliferation by MTT assay. RESULTS: Low and high concentrations of genistein induced an ERK1/2-independent decrease in phosphorylated p90RSK. This effect was accompanied by decreased cell proliferation at high concentrations and an increased response at low concentrations of genistein following a 48-hour exposure. CONCLUSION: Concentration-dependent actions of genistein in T47D cells may be due to differential activation of signaling molecules.
Authors: Nicholas Theodosakis; Goran Micevic; Casey G Langdon; Alessandra Ventura; Robert Means; David F Stern; Marcus W Bosenberg Journal: J Invest Dermatol Date: 2017-06-07 Impact factor: 8.551
Authors: Benjamin A Mooso; Ruth L Vinall; Clifford G Tepper; Rosalinda M Savoy; Jean P Cheung; Sheetal Singh; Salma Siddiqui; Yu Wang; Roble G Bedolla; Anthony Martinez; Maria Mudryj; Hsing-Jien Kung; Ralph W Devere White; Paramita M Ghosh Journal: Endocr Relat Cancer Date: 2012-11-09 Impact factor: 5.678
Authors: Han Sang Kim; Su-Jin Kim; Jinhyung Bae; Yiyi Wang; Sun Young Park; Young Sil Min; Hyun Dong Je; Uy Dong Sohn Journal: Korean J Physiol Pharmacol Date: 2016-10-28 Impact factor: 2.016