PURPOSE: Analyze the influence of low-intensity laser therapy in the sciatic nerve regeneration of rats submitted to controlled crush through histological analysis. METHODS: Were used 20 Wistar rats, to analyze the influence of low-intensity laser therapy in the sciatic nerve regeneration, where the injury of the type axonotmesis was induced by a haemostatic clamp Crile (2nd level of the rack). The animals were randomly distributed in 2 groups. Control group (CG n = 10) and Laser group (LG n = 10). These were subdivided in 2 subgroups each, according to the euthanasia period: (CG14 - n = 5 and CG21 - n = 5) and (LG14 - n = 5 and LG21 - n = 5). At the end of treatment, the samples were removed and prepared for histological analysis, where were analyzed and quantified the following findings: Schwann cells, myelinic axons with large diameter and neurons. RESULTS: In the groups submitted to low-intensity laser therapy, were observed an increase in the number of all analyzed aspects with significance level. CONCLUSION: The irradiation with low intensity laser (904nm) influenced positively the regeneration of the sciatic nerve in Wistar rats after being injured by crush (axonotmesis), becoming the nerve recovery more rapid and efficient.
PURPOSE: Analyze the influence of low-intensity laser therapy in the sciatic nerve regeneration of rats submitted to controlled crush through histological analysis. METHODS: Were used 20 Wistar rats, to analyze the influence of low-intensity laser therapy in the sciatic nerve regeneration, where the injury of the type axonotmesis was induced by a haemostatic clamp Crile (2nd level of the rack). The animals were randomly distributed in 2 groups. Control group (CG n = 10) and Laser group (LG n = 10). These were subdivided in 2 subgroups each, according to the euthanasia period: (CG14 - n = 5 and CG21 - n = 5) and (LG14 - n = 5 and LG21 - n = 5). At the end of treatment, the samples were removed and prepared for histological analysis, where were analyzed and quantified the following findings: Schwann cells, myelinic axons with large diameter and neurons. RESULTS: In the groups submitted to low-intensity laser therapy, were observed an increase in the number of all analyzed aspects with significance level. CONCLUSION: The irradiation with low intensity laser (904nm) influenced positively the regeneration of the sciatic nerve in Wistar rats after being injured by crush (axonotmesis), becoming the nerve recovery more rapid and efficient.
Authors: Renata Ferreira de Oliveira; Daniela Miranda Richarte de Andrade Salgado; Lívia Tosi Trevelin; Raquel Marianna Lopes; Sandra Ribeiro Barros da Cunha; Ana Cecília Correa Aranha; Carlos de Paula Eduardo; Patricia Moreira de Freitas Journal: Lasers Med Sci Date: 2014-02-12 Impact factor: 3.161
Authors: L Andreo; C B Soldera; B G Ribeiro; P R V de Matos; S K Bussadori; K P S Fernandes; R A Mesquita-Ferrari Journal: Lasers Med Sci Date: 2017-10-23 Impact factor: 3.161
Authors: Daniel de Oliveira Martins; Fabio Martinez dos Santos; Mara Evany de Oliveira; Luiz R G de Britto; José Benedito Dias Lemos; Marucia Chacur Journal: J Neurotrauma Date: 2013-03-20 Impact factor: 5.269
Authors: Fernando José Dias; João Paulo Mardegan Issa; Mamie Mizusaki Iyomasa; Joaquim Coutinho-Netto; Ricardo Alexandre Junqueria Calzzani; Daniela Mizusaki Iyomasa; Luiz Gustavo Sousa; Sonia Regina Yokomizo de Almeida; Diego Pulzatto Cury; Ii-sei Watanabe Journal: Biomed Res Int Date: 2013-07-02 Impact factor: 3.411