Effects of neonatal sympathectomy with 6-hydroxydopamine or guanethidine on survival of neurons in the intermediolateral cell column of rat spinal cord.

The effects of removing target cells on survival of, and inputs to, sympathetic preganglionic neurons were studied in rats that were sympathectomized with 6-hydroxydopamine (6-OHDA) or guanethidine sulfate. Separate groups of neonatal and 1-week male rats were given injections of 6-OHDA for 10 days and of guanethidine for 3 weeks (5 days/week), respectively. Histofluorescence results suggest that catecholaminergic neurons in most ganglia are destroyed with treatment except for adrenal medulla, which is unaffected [14], and the pelvic ganglion where only partial destruction occurs. Cells in the intermediolateral cell column from representative spinal cord segments of treated and control adult rats were counted. In 6-OHDA-treated rats, cells decreased in number in all segments compared to controls. In guanethidine-treated rats, cells were also decreased in number; in some segments the decrease was significantly greater than with 6-OHDA. Sympathectomy had no effect on neurons in the intermediate gray of L5 or in the ventral horn of T3. The results of this study demonstrate that peripheral sympathectomy causes loss of sympathetic preganglionic neurons and that guanethidine is slightly more effective than 6-OHDA.