BACKGROUND: Thrombophilias have been suggested as a possible cause of recurrent pregnancy loss (RPL). OBJECTIVE: Testing for the association of factor V Leiden (FVL) and prothrombin (FII) mutations with RPL among cases from the Nile Delta region of Egypt. SUBJECTS AND METHODS: Participants included 72 cases having a history of two or more events of unexplained RPL and 70 controls with a good obstetric history. Detection of FVL (G1691A) and FII (G20210A) mutations was carried out using PCR with sequence specific primers. RESULTS: Cases showed a significantly higher frequency of FVL GA (OR = 21·38, P<0·0001) and FII GA (OR = 36·7, P<0·0001) genotypes. Cases with two or more risk factors had significant higher frequency of both mutant genotypes, while no significant difference could be elicited related to primary or secondary infertility, number of fetal losses, or phase of pregnancy loss. CONCLUSION: Screening for thrombophilic mutations may help in the prevention of unexplained RPL.
BACKGROUND:Thrombophilias have been suggested as a possible cause of recurrent pregnancy loss (RPL). OBJECTIVE: Testing for the association of factor V Leiden (FVL) and prothrombin (FII) mutations with RPL among cases from the Nile Delta region of Egypt. SUBJECTS AND METHODS: Participants included 72 cases having a history of two or more events of unexplained RPL and 70 controls with a good obstetric history. Detection of FVL (G1691A) and FII (G20210A) mutations was carried out using PCR with sequence specific primers. RESULTS: Cases showed a significantly higher frequency of FVL GA (OR = 21·38, P<0·0001) and FII GA (OR = 36·7, P<0·0001) genotypes. Cases with two or more risk factors had significant higher frequency of both mutant genotypes, while no significant difference could be elicited related to primary or secondary infertility, number of fetal losses, or phase of pregnancy loss. CONCLUSION: Screening for thrombophilic mutations may help in the prevention of unexplained RPL.
Authors: Nasir A S Al-Allawi; Maida Y Shamdeen; Qais O Mohammed; Ahmed S Ahmed Journal: Indian J Hematol Blood Transfus Date: 2014-02-23 Impact factor: 0.900