BACKGROUND AND AIM: We recently showed that L-carnitine reduced oxidative stress and suppressed energy metabolism, while α-tocopherol only prevented redox imbalance, in the obstructed kidney of rats subjected to 24-hr of unilateral ureteral obstruction (UUO). The present study was undertaken to investigate the effects of both compounds on disturbed renal hemodynamics, solutes-excretion, and urine-concentrating ability as well as renal oxidative stress and suppressed metabolism at early hours following release of 24-hr UUO. METHODS: UUO was induced in anaesthetized rats that received L-carnitine, α-tocopherol or their vehicles in four different groups. Each rat was re-anaesthetized, prepared for renal functional measurements, and its ureteral obstruction was released at 24-hr. Then, urines of both kidneys were separately collected during 30-90 min of post-release, with taking blood samples at beginning and end of it. Finally, both kidneys were removed and preserved at -80°C for future measuring their levels of ATP and ADP as well as malondialdehyde (MDA) and ferric reducing/antioxidant power (FRAP). There were also sham and control groups. RESULTS: Post-obstructed kidney (POK) of vehicle-treated groups compared to equivalent kidney of sham group had lower ATP, ATP/ADP, FRAP, creatinine clearance, absolute Na(+)- and K(+)-excretion, and effective free-water reabsorption, but higher MDA and ADP. L-carnitine could improve oxidative stress and suppressed energy metabolism and α-tocopherol normalized redox state, but both compounds did not have any effects on altered functional variables of the POK. CONCLUSION: Oxidative stress and suppressed energy metabolism may not be involved in the development of renal dysfunction during acute ureteral obstruction.
BACKGROUND AND AIM: We recently showed that L-carnitine reduced oxidative stress and suppressed energy metabolism, while α-tocopherol only prevented redox imbalance, in the obstructed kidney of rats subjected to 24-hr of unilateral ureteral obstruction (UUO). The present study was undertaken to investigate the effects of both compounds on disturbed renal hemodynamics, solutes-excretion, and urine-concentrating ability as well as renal oxidative stress and suppressed metabolism at early hours following release of 24-hr UUO. METHODS: UUO was induced in anaesthetized rats that received L-carnitine, α-tocopherol or their vehicles in four different groups. Each rat was re-anaesthetized, prepared for renal functional measurements, and its ureteral obstruction was released at 24-hr. Then, urines of both kidneys were separately collected during 30-90 min of post-release, with taking blood samples at beginning and end of it. Finally, both kidneys were removed and preserved at -80°C for future measuring their levels of ATP and ADP as well as malondialdehyde (MDA) and ferric reducing/antioxidant power (FRAP). There were also sham and control groups. RESULTS: Post-obstructed kidney (POK) of vehicle-treated groups compared to equivalent kidney of sham group had lower ATP, ATP/ADP, FRAP, creatinine clearance, absolute Na(+)- and K(+)-excretion, and effective free-water reabsorption, but higher MDA and ADP. L-carnitine could improve oxidative stress and suppressed energy metabolism and α-tocopherol normalized redox state, but both compounds did not have any effects on altered functional variables of the POK. CONCLUSION: Oxidative stress and suppressed energy metabolism may not be involved in the development of renal dysfunction during acute ureteral obstruction.
Authors: Hai Yan Zhao; Hui Ying Li; Jian Jin; Ji Zhe Jin; Long Ye Zhang; Mei Ying Xuan; Xue Mei Jin; Yu Ji Jiang; Hai Lan Zheng; Ying Shun Jin; Yong Jie Jin; Bum Soon Choi; Chul Woo Yang; Shang Guo Piao; Can Li Journal: Korean J Intern Med Date: 2020-09-18 Impact factor: 2.884