Literature DB >> 2126169

Transformation of Pseudomonas aeruginosa by electroporation.

J M Diver1, L E Bryan, P A Sokol.   

Abstract

Optimum conditions were defined for the electrotransformation of Pseudomonas aeruginosa PAO1 with plasmid pLAFR1, resulting in a 1500-fold increase in transformation efficiency compared to conventional chemical transformation with MgCl2. In addition, PAO236 and two out of three recent clinical isolates of P. aeruginosa from the sputum of cystic fibrosis patients were successfully transformed with plasmid pUC19 1.8. The applied voltage and the electroporation buffer composition were shown to have the greatest effect on transformation efficiency. Freezing the cells and prolonged storage at -70 degrees C did not significantly affect the transformation efficiency. The clinical isolates tested had lower transformation efficiencies than PAO1.

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Year:  1990        PMID： 2126169     DOI： 10.1016/0003-2697(90)90046-c

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  16 in total

1.  Molecular characterization of MexL, the transcriptional repressor of the mexJK multidrug efflux operon in Pseudomonas aeruginosa.

Authors:  Rungtip Chuanchuen; Jared B Gaynor; RoxAnn Karkhoff-Schweizer; Herbert P Schweizer
Journal:  Antimicrob Agents Chemother       Date:  2005-05       Impact factor: 5.191

2.  Use of electroporation to construct isogenic mutants of Haemophilus ducreyi.

Authors:  E J Hansen; J L Latimer; S E Thomas; M Helminen; W L Albritton; J D Radolf
Journal:  J Bacteriol       Date:  1992-08       Impact factor: 3.490

3.  Persistence mechanisms in Pseudomonas aeruginosa from cystic fibrosis patients undergoing ciprofloxacin therapy.

Authors:  J M Diver; T Schollaardt; H R Rabin; C Thorson; L E Bryan
Journal:  Antimicrob Agents Chemother       Date:  1991-08       Impact factor: 5.191

4.  Pseudomonas aeruginosa possesses two putative type I signal peptidases, LepB and PA1303, each with distinct roles in physiology and virulence.

Authors:  Richard D Waite; Ruth S Rose; Minnie Rangarajan; Joseph Aduse-Opoku; Ahmed Hashim; Michael A Curtis
Journal:  J Bacteriol       Date:  2012-06-22       Impact factor: 3.490

5.  Posttranslational control of the algT (algU)-encoded sigma22 for expression of the alginate regulon in Pseudomonas aeruginosa and localization of its antagonist proteins MucA and MucB (AlgN).

Authors:  K Mathee; C J McPherson; D E Ohman
Journal:  J Bacteriol       Date:  1997-06       Impact factor: 3.490

6.  Physiological function of soluble cytochrome c-552 from alkaliphilic Pseudomonas alcaliphila AL15-21(T).

Authors:  Toshihede Matsuno; Kazuaki Yoshimune; Isao Yumoto
Journal:  J Bioenerg Biomembr       Date:  2011-07-16       Impact factor: 2.945

7.  Design and study of the efflux function of the EGFP fused MexAB-OprM membrane transporter in Pseudomonas aeruginosa using fluorescence spectroscopy.

Authors:  Feng Ding; Kerry J Lee; Ardeschir Vahedi-Faridi; Hiroshi Yoneyama; Christopher J Osgood; Xiao-Hong Nancy Xu
Journal:  Analyst       Date:  2014-04-29       Impact factor: 4.616

8.  lfnA from Pseudomonas aeruginosa O12 and wbuX from Escherichia coli O145 encode membrane-associated proteins and are required for expression of 2,6-dideoxy-2-acetamidino-L-galactose in lipopolysaccharide O antigen.

Authors:  Jerry D King; Erin F Mulrooney; Evgeny Vinogradov; Bernd Kneidinger; Kristen Mead; Joseph S Lam
Journal:  J Bacteriol       Date:  2007-12-21       Impact factor: 3.490

9.  A Simple and Rapid Method of Transformation of Streptomyces rimosus R6 and Other Streptomycetes by Electroporation.

Authors:  J Pigac; H Schrempf
Journal:  Appl Environ Microbiol       Date:  1995-01       Impact factor: 4.792

10.  FptA, the Fe(III)-pyochelin receptor of Pseudomonas aeruginosa: a phenolate siderophore receptor homologous to hydroxamate siderophore receptors.

Authors:  R G Ankenbauer; H N Quan
Journal:  J Bacteriol       Date:  1994-01       Impact factor: 3.490
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