Sphingosine-1-phosphate aggravates antigen-induced airway inflammation in mice.

UNLABELLED: Recent investigations suggest an involvement of sphingosine-1-phosphate (S1P) in the pathogenesis of allergic bronchial asthma. However, the role of S1P in the development of asthma is still controversial. Our aim was to investigate the effects of intranasal application of S1P on antigen-induced airway inflammation in a mouse model of allergic bronchial asthma.
METHODOLOGY: Male BALB/c mice were actively sensitized with ovalbumin antigen, and were repeatedly challenged with aerosolized antigen. Animals also received an intranasal administration of S1P (10-5 M, 20 µL) or its vehicle (1% methanol in sterile PBS, 20 µL) 30 min prior to each antigen challenge. Histological examinations of the lungs and determination of cell number in the bronchoalveolar lavage fluids (BALFs) were studied.
RESULTS: The airway inflammation induced by antigen exposure was significantly augmented by the intranasal administration of S1P: the cell number in BALFs of the S1P-treated, antigen-challenged mice (S1P-Challenged, 48.9±4.8 x 10(4)/mL BALF) was significantly increased as compared with those of the vehicle-treated, antigen-challenged ones (Vehicle-Challenged, 26.3±5.7 x 10(4)/mL BALF, P<0.01).
CONCLUSION: In mice, the intranasal administration of S1P might aggravate the antigen-induced airway inflammation.

INTRODUCTION

The dramatic increase in the number of asthma cases over the last decades is of great concern for public health in the world [1]. Allergic bronchial asthma is characterized by structural and functional abnormalities of the bronchial epithelium, accumulation of inflammatory cells in the bronchial mucosa, remodeling of the airway tissue structure, and airway hyperresponsiveness. Many mediators have been identified that play significant roles in the initiation and progression of the disease, but the exact mechanism of the pathogenesis of asthma is still unclear.

Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that mediates diverse biological responses [2-6]. Recently, an involvement of S1P in allergic bronchial asthma has been suggested [7-13]. Ammit and colleagues [7] firstly demonstrated that S1P levels are elevated in the airways of individuals with asthma after segmental allergen challenge. The finding that S1P can act as a chemotactic agent for eosinophils further suggests an involvement of S1P in pathophysiology of asthma [8]. Indeed, inhalation of inhibitors for sphingosine kinase (SphK), which produces S1P directly from sphingosine, attenuated antigen-induced airway inflammation in mice [9]. In addition, S1P might have an ability to cause airway hyperresponsiveness [10-13], one of the characteristic features of allergic bronchial asthma. Contrary to these observations, an intratracheal instillation of FTY720, an S1P receptor agonist, prevented antigen-induced airway inflammation and hyperresponsiveness in mice [14]. Thus, the role of S1P in the development of asthma is still controversial.

In the present study, the effects of pretreatment with S1P by intranasal administration on antigen-induced inflammatory signs of the airways were determined in a murine model of allergic bronchial asthma.

METHODS

Animals and Treatments

Male BALB/c mice were purchased from the Charles River Japan, Inc. (Kanagawa, Japan) and housed in a pathogen-free facility. All animal experiments were approved by the Animal Care Committee of the Hoshi University (Tokyo, Japan).

Preparation of a murine model of allergic bronchial asthma, which has an in vivo AHR [15], was performed as described previously [16-20]. In brief, BALB/c mice (8 weeks of age) were actively sensitized by intraperitoneal injections of 8 µg ovalbumin (OA; Seikagaku Co., Tokyo, Japan) with 2 mg Imject Alum (Pierce Biotechnology, Inc., Rockfold, IL, USA) on Day 0 and Day 5. The sensitized mice were challenged with aerosolized OA-saline solution (5 mg/mL) for 30 min on Days 12, 16 and 20. The OA aerosol was generated with an ultrasonic nebulizer (Nihon Kohden, Tokyo, Japan) and introduced to a Plexiglas chamber box (130 x 200 mm, 100 mm height) in which the mice were placed. The animals also received intranasal administration of S1P (10-5 M, 20 µL) or its vehicle (1% methanol in sterile PBS, 20 µL) 30 min prior to each antigen challenge by the method previously described [18]. The dose of S1P was decided based on the previous report [7]. Twenty-four hours after the last OA challenge, mice were sacrificed by exsanguination from abdominal aorta under urethane (1.6 g/kg, i.p.; Sigma and Aldrich, St. Louis, MO) anesthesia. Then histologic examination and cell count in bronchoalveolar lavage (BAL) fluid were carried out by the methods previously described [18].

Statistical Analyses

The cell counts data were expressed as the mean with S.E. Statistical significance of difference was determined by unpaired Student's t-test or two-way analysis of variance (ANOVA) with post hoc Bonferroni/Dunn (StatView for Macintosh ver. 5.0, SAS Institute, Inc., NC). A value of p<0.05 was considered significant.

RESULTS AND DISCUSSION

In the present study, we used our well-established murine model of allergic bronchial asthma [16-20]. Histochemical examination using hematoxylin and eosin staining revealed a marked lung inflammation in the repeatedly antigen-challenged mice (Fig. ) as compared with the control animals (Fig. ): a marked infiltration of inflammatory cells, mainly eosinophils, was observed in the lungs of the antigen-challenged mice. The inflammation score determined as previously described [19] was significantly increased in lungs of the antigen-challenged mice (2.4±0.4) than that of the control animals (0.9±0.3, P<0.05). To further quantify the airway inflammation, cell counts in bronchoalveolar lavage (BAL) fluids were carried out. As shown in Fig. (), the cell counts in BAL fluids of the repeatedly antigen- challenged mice were significantly increased as compared with those of the control group (Fig. , Vehicle-Control vs Vehicle-Challenged groups; P<0.05). As previously reported [19, 20], most of the increased cells were eosinophils. The vehicle used had no effect on baseline lung histology and BAL cell counts and the airway inflammation induced by antigen exposure (data not shown).

Recently, an involvement of sphingosine-1-phosphate (S1P) in the pathogenesis of allergic bronchial asthma has been suggested by the fact that S1P levels are elevated in the airways of asthmatics [7]. The current study revealed that, although the S1P administration alone had no effect on the lung histology (Fig. , ) and the BAL cell counts in control animals (Fig. , Vehicle-Control vs S1P-Control), administration of S1P prior to each antigen challenge aggravated the antigen-induced increase in BAL cell counts in mice (Fig. , Vehicle-Challenged vs S1P-Challenged groups; P<0.01). Different cell counts using Diff-Quik staining [19] revealed that the cell composition in BAL fluids of the S1P-Challenged group (macrophages: 47.1±9.9%, eosinophils: 38.6±6.2%, lymphocytes: 12.1±2.5%, and neutrophils: 2.2±1.1%) was similar to that of the Vehicle-Challenged one (48.9±10.1%, 37.8±5.6%,11.1±2.2% and 2.2±1.2%, respectively). An increase in the inflammation score was also observed in the lungs of the S1P-Challenged group (2.8±0.5), although the difference was not statistically significant when compared with that of the Vehicle-Challenged one (2.4±0.4, P>0.05). The results that S1P aggravated antigen-induced airway inflammation might be consistent with the previous report that pharmacological inhibition of sphingosine kinase (SphK) attenuated airway inflammation in mouse experimental asthma [9]. It is thus possible that endogenously released S1P is one of the mediators that play significant roles in the initiation and/or progression of allergic bronchial asthma.

Contrary to our results, Idzko and colleagues [14] reported that an intratracheal instillation of FTY720, an S1P receptor agonist, or S1P itself prior to each antigen inhalation abolished inflammatory cells infiltration into the airways in the BALB/c mice. Although the reason for the discrepancy is not known for certain, differences in the experimental conditions, such as difference in the protocols for antigen sensitization and inhalation, difference in the concentration and volume of S1P solution (20 µL of 10-5 M currently vs 80 µL of 10-6 M in their study), and/or difference in the administration route of S1P (intranasally vs intratracheally, respectively), might be involved in it. While S1P and/or S1P receptor agonists, such as FTY720, have been suggested as a novel therapeutic strategy for airway inflammation [14, 21], further studies are required for making clear the exact role of S1P in the pathogenesis of allergic bronchial asthma.

In summary, the intranasal application of S1P caused an aggravation of airway inflammation induced by antigen exposure in a mouse model of allergic bronchial asthma. The results might raise a caution for clinical use of S1P receptor agonists, such as FTY720, in asthma treatment.

CONFLICT OF INTEREST STATEMENT

None of the authors have a financial relationship with a commercial entity that has an interest in the subject of this manuscript.