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Literature DB >> 21255554

Visualization of ceramide channels by transmission electron microscopy.

Soumya Samanta¹, Johnny Stiban, Timothy K Maugel, Marco Colombini.

Abstract

Functional studies have shown that the sphingolipid ceramide, self-assembles in phospholipid membranes to form large channels capable of allowing proteins to cross the membrane. Here these channels are visualized by negative stain transmission electron microscopy. The images contain features consistent with stain-filled pores having a roughly circular profile. There is no indication of tilt, and the results are consistent with the formation of right cylinders. The sizes of the pores range from 5 to 40nm in diameter with an asymmetric distribution indicating no apparent upper size limit. The size distribution matches well with the distribution of sizes calculated from electrophysiological measurements.

Entities: CellLine Chemical Disease Gene Species

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Year: 2011 PMID： 21255554 PMCID： PMC3062739 DOI： 10.1016/j.bbamem.2011.01.007

Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002

20 in total

1. The lipids C2- and C16-ceramide form large stable channels. Implications for apoptosis.

Authors: L J Siskind; M Colombini
Journal: J Biol Chem Date: 2000-12-08 Impact factor: 5.157

Review 2. Regulation of ceramide channels by Bcl-2 family proteins.

Authors: Vidyaramanan Ganesan; Marco Colombini
Journal: FEBS Lett Date: 2010-02-14 Impact factor: 4.124

3. Characterization of channels isolated from plant mitochondria.

Authors: M Colombini
Journal: Methods Enzymol Date: 1987 Impact factor: 1.600

4. Formation of bimolecular membranes from lipid monolayers and a study of their electrical properties.

Authors: M Montal; P Mueller
Journal: Proc Natl Acad Sci U S A Date: 1972-12 Impact factor: 11.205

5. Ceramide channels increase the permeability of the mitochondrial outer membrane to small proteins.

Authors: Leah J Siskind; Richard N Kolesnick; Marco Colombini
Journal: J Biol Chem Date: 2002-05-10 Impact factor: 5.157

6. Mass spectrometric identification of increased C16 ceramide levels during apoptosis.

Authors: R L Thomas; C M Matsko; M T Lotze; A A Amoscato
Journal: J Biol Chem Date: 1999-10-22 Impact factor: 5.157

7. Mitochondrial lipid alterations during Fas- and radiation-induced apoptosis.

Authors: C M Matsko; O C Hunter; H Rabinowich; M T Lotze; A A Amoscato
Journal: Biochem Biophys Res Commun Date: 2001-10-12 Impact factor: 3.575

8. Direct effect of ceramide on the mitochondrial electron transport chain leads to generation of reactive oxygen species. Role of mitochondrial glutathione.

Authors: C García-Ruiz; A Colell; M Marí; A Morales; J C Fernández-Checa
Journal: J Biol Chem Date: 1997-04-25 Impact factor: 5.157

9. Mitochondrial ceramide increases in UV-irradiated HeLa cells and is mainly derived from hydrolysis of sphingomyelin.

Authors: Qiang Dai; Jihua Liu; Jun Chen; David Durrant; Thomas M McIntyre; Ray M Lee
Journal: Oncogene Date: 2004-04-29 Impact factor: 9.867

10. Enlargement and contracture of C2-ceramide channels.

Authors: Leah J Siskind; Amirparviz Davoody; Naomi Lewin; Stephanie Marshall; Marco Colombini
Journal: Biophys J Date: 2003-09 Impact factor: 4.033

15 in total

Review 1. Ceramide channels and mitochondrial outer membrane permeability.

Authors: Marco Colombini
Journal: J Bioenerg Biomembr Date: 2016-01-22 Impact factor: 2.945

Review 2. Nuclear sphingolipid metabolism.

Authors: Natasha C Lucki; Marion B Sewer
Journal: Annu Rev Physiol Date: 2011-09-09 Impact factor: 19.318

Visualization of ceramide channels by transmission electron microscopy.

1. The lipids C2- and C16-ceramide form large stable channels. Implications for apoptosis.

Review 2. Regulation of ceramide channels by Bcl-2 family proteins.

3. Characterization of channels isolated from plant mitochondria.

4. Formation of bimolecular membranes from lipid monolayers and a study of their electrical properties.

5. Ceramide channels increase the permeability of the mitochondrial outer membrane to small proteins.

6. Mass spectrometric identification of increased C16 ceramide levels during apoptosis.

7. Mitochondrial lipid alterations during Fas- and radiation-induced apoptosis.

8. Direct effect of ceramide on the mitochondrial electron transport chain leads to generation of reactive oxygen species. Role of mitochondrial glutathione.

9. Mitochondrial ceramide increases in UV-irradiated HeLa cells and is mainly derived from hydrolysis of sphingomyelin.

10. Enlargement and contracture of C2-ceramide channels.

Review 1. Ceramide channels and mitochondrial outer membrane permeability.

Review 2. Nuclear sphingolipid metabolism.

3. Ceramide channels: influence of molecular structure on channel formation in membranes.

Review 4. Ceramide synthases at the centre of sphingolipid metabolism and biology.

Review 5. Sphingolipids and mitochondrial apoptosis.

Review 6. Advances in determining signaling mechanisms of ceramide and role in disease.

7. Ceramide channel: Structural basis for selective membrane targeting.

8. Ceramide formation mediated by acid sphingomyelinase facilitates endosomal escape of caliciviruses.

9. Ceramide channels: destabilization by Bcl-xL and role in apoptosis.

10. Dynamics of ceramide channels detected using a microfluidic system.