Literature DB >> 2125292

An efficient method for isolation of plasmid DNA from methylotrophic bacteria.

V Brenner1, I Holubová, J Hubácek.   

Abstract

A method, suitable for the isolation of closed circular plasmid DNA from methylotrophic bacteria is described. Improvement of cell lysis was achieved by butanol extraction of cells before application of the lytic agent. Using this method, cryptic plasmids of 7.8, 14, 36 and 200 kb were purified from soil-isolated methylotrophs.

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Year:  1990        PMID： 2125292     DOI： 10.1007/bf02821415

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  6 in total

1.  Methylotrophic bacteria: biochemical diversity and genetics.

Authors:  C L Haber; L N Allen; S Zhao; R S Hanson
Journal:  Science       Date:  1983-09-16       Impact factor: 47.728

2.  DNA:DNA hybridization studies on the pink-pigmented facultative methylotrophs.

Authors:  D W Hood; C S Dow; P N Green
Journal:  J Gen Microbiol       Date:  1987-03

3.  A simple method for the qualitative demonstration of large and small plasmid deoxyribonucleic acids in gram-positive and gram-negative bacteria.

Authors:  J M Ranhand
Journal:  Anal Biochem       Date:  1982-05-15       Impact factor: 3.365

4.  Rapid procedure for detection and isolation of large and small plasmids.

Authors:  C I Kado; S T Liu
Journal:  J Bacteriol       Date:  1981-03       Impact factor: 3.490

5.  Genetic and molecular characterization of the Pseudomonas plasmid pVS1.

Authors:  Y Itoh; J M Watson; D Haas; T Leisinger
Journal:  Plasmid       Date:  1984-05       Impact factor: 3.466

6.  Plasmids in methanotrophic bacteria: isolation, characterization and DNA hybridization analysis.

Authors:  M E Lidstrom; A E Wopat
Journal:  Arch Microbiol       Date:  1984-11       Impact factor: 2.552
  6 in total
  2 in total

1.  Characterization of new plasmids from methylotrophic bacteria.

Authors:  V Brenner; I Holubová; O Benada; J Hubácek
Journal:  Antonie Van Leeuwenhoek       Date:  1991-07       Impact factor: 2.271

2.  Intergeneric conjugal transfer of Escherichia coli/Methylobacterium sp. shuttle vector.

Authors:  I Holubová; V Brenner; J Hubácek
Journal:  Folia Microbiol (Praha)       Date:  1993       Impact factor: 2.099
  2 in total

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