Literature DB >> 2124698

Mutants of Saccharomyces cerevisiae defective in the farnesylation of Ras proteins.

L E Goodman1, S R Judd, C C Farnsworth, S Powers, M H Gelb, J A Glomset, F Tamanoi.   

Abstract

Ras proteins are post-translationally modified by farnesylation. In the present investigation, we identified an activity in crude soluble extracts of yeast cells that catalyzes the transfer of a farnesyl moiety from farnesyl pyrophosphate to yeast RAS2 protein. RAS2 proteins having a C-terminal Cys-Ali-Ali-Xaa sequence (where Ali is an aliphatic amino acid and Xaa is the unspecified C-terminal amino acid) served as substrates for this reaction, whereas RAS2 proteins with an altered or deleted Cys-Ali-Ali-Xaa sequence did not. A yeast mutant, dpr1/ram1, originally isolated as a Ras-processing mutant was shown to be defective in farnesyltransferase activity. In addition, another mutant, ram2, also was defective in the transferase activity. These results demonstrate that at least two genes, DPR1/RAM1 and RAM2, are required for the farnesyltransferase activity in yeast.

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Year:  1990        PMID： 2124698      PMCID： PMC55233          DOI： 10.1073/pnas.87.24.9665

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  24 in total

1.  Common modifications of trimeric G proteins and ras protein: involvement of polyisoprenylation.

Authors:  A A Finegold; W R Schafer; J Rine; M Whiteway; F Tamanoi
Journal:  Science       Date:  1990-07-13       Impact factor: 47.728

2.  Inhibition of purified p21ras farnesyl:protein transferase by Cys-AAX tetrapeptides.

Authors:  Y Reiss; J L Goldstein; M C Seabra; P J Casey; M S Brown
Journal:  Cell       Date:  1990-07-13       Impact factor: 41.582

3.  G protein gamma subunits contain a 20-carbon isoprenoid.

Authors:  S M Mumby; P J Casey; A G Gilman; S Gutowski; P C Sternweis
Journal:  Proc Natl Acad Sci U S A       Date:  1990-08       Impact factor: 11.205

4.  Differential activation of yeast adenylate cyclase by wild-type and mutant RAS proteins.

Authors:  D Broek; N Samiy; O Fasano; A Fujiyama; F Tamanoi; J Northup; M Wigler
Journal:  Cell       Date:  1985-07       Impact factor: 41.582

5.  RAS2 of Saccharomyces cerevisiae is required for gluconeogenic growth and proper response to nutrient limitation.

Authors:  K Tatchell; L C Robinson; M Breitenbach
Journal:  Proc Natl Acad Sci U S A       Date:  1985-06       Impact factor: 11.205

6.  Enzymatic coupling of cholesterol intermediates to a mating pheromone precursor and to the ras protein.

Authors:  W R Schafer; C E Trueblood; C C Yang; M P Mayer; S Rosenberg; C D Poulter; S H Kim; J Rine
Journal:  Science       Date:  1990-09-07       Impact factor: 47.728

7.  RAM, a gene of yeast required for a functional modification of RAS proteins and for production of mating pheromone a-factor.

Authors:  S Powers; S Michaelis; D Broek; S Santa Anna; J Field; I Herskowitz; M Wigler
Journal:  Cell       Date:  1986-11-07       Impact factor: 41.582

8.  Processing and fatty acid acylation of RAS1 and RAS2 proteins in Saccharomyces cerevisiae.

Authors:  A Fujiyama; F Tamanoi
Journal:  Proc Natl Acad Sci U S A       Date:  1986-03       Impact factor: 11.205

9.  Posttranslational modification of ras proteins: detection of a modification prior to fatty acid acylation and cloning of a gene responsible for the modification.

Authors:  F Tamanoi; E C Hsueh; L E Goodman; A R Cobitz; R J Detrick; W R Brown; A Fujiyama
Journal:  J Cell Biochem       Date:  1988-03       Impact factor: 4.429

10.  A novel yeast mutant defective in the processing of ras proteins: assessment of the effect of the mutation on processing steps.

Authors:  A Fujiyama; K Matsumoto; F Tamanoi
Journal:  EMBO J       Date:  1987-01       Impact factor: 11.598
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  35 in total

1.  Acylation stabilizes a protease-resistant conformation of protoporphyrinogen oxidase, the molecular target of diphenyl ether-type herbicides.

Authors:  S Arnould; M Takahashi; J M Camadro
Journal:  Proc Natl Acad Sci U S A       Date:  1999-12-21       Impact factor: 11.205

2.  A protein geranylgeranyltransferase from bovine brain: implications for protein prenylation specificity.

Authors:  K Yokoyama; G W Goodwin; F Ghomashchi; J A Glomset; M H Gelb
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-15       Impact factor: 11.205

3.  Protein geranylgeranyltransferase of Saccharomyces cerevisiae is specific for Cys-Xaa-Xaa-Leu motif proteins and requires the CDC43 gene product but not the DPR1 gene product.

Authors:  A A Finegold; D I Johnson; C C Farnsworth; M H Gelb; S R Judd; J A Glomset; F Tamanoi
Journal:  Proc Natl Acad Sci U S A       Date:  1991-05-15       Impact factor: 11.205

4.  Rho2 is a target of the farnesyltransferase Cpp1 and acts upstream of Pmk1 mitogen-activated protein kinase signaling in fission yeast.

Authors:  Yan Ma; Takayoshi Kuno; Ayako Kita; Yuta Asayama; Reiko Sugiura
Journal:  Mol Biol Cell       Date:  2006-09-27       Impact factor: 4.138

5.  Phosphatidylinositol 3-kinase mediates epidermal growth factor-induced activation of the c-Jun N-terminal kinase signaling pathway.

Authors:  S K Logan; M Falasca; P Hu; J Schlessinger
Journal:  Mol Cell Biol       Date:  1997-10       Impact factor: 4.272

6.  Cloning and expression of a cDNA encoding the alpha subunit of rat p21ras protein farnesyltransferase.

Authors:  W J Chen; D A Andres; J L Goldstein; M S Brown
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-15       Impact factor: 11.205

7.  Mutant farnesyltransferase beta subunit of Saccharomyces cerevisiae that can substitute for geranylgeranyltransferase type I beta subunit.

Authors:  H Mitsuzawa; K Esson; F Tamanoi
Journal:  Proc Natl Acad Sci U S A       Date:  1995-02-28       Impact factor: 11.205

8.  Ras membrane targeting is essential for glucose signaling but not for viability in yeast.

Authors:  S Bhattacharya; L Chen; J R Broach; S Powers
Journal:  Proc Natl Acad Sci U S A       Date:  1995-03-28       Impact factor: 11.205

9.  AIPL1, a protein implicated in Leber's congenital amaurosis, interacts with and aids in processing of farnesylated proteins.

Authors:  Visvanathan Ramamurthy; Melanie Roberts; Focco van den Akker; Gregory Niemi; T A Reh; James B Hurley
Journal:  Proc Natl Acad Sci U S A       Date:  2003-10-10       Impact factor: 11.205

10.  Genetic evidence for in vivo cross-specificity of the CaaX-box protein prenyltransferases farnesyltransferase and geranylgeranyltransferase-I in Saccharomyces cerevisiae.

Authors:  C E Trueblood; Y Ohya; J Rine
Journal:  Mol Cell Biol       Date:  1993-07       Impact factor: 4.272
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