Literature DB >> 2124546

Sialylated carbohydrate chains of recombinant human glycoproteins expressed in Chinese hamster ovary cells contain traces of N-glycolylneuraminic acid.

C H Hokke1, A A Bergwerff, G W van Dedem, J van Oostrum, J P Kamerling, J F Vliegenthart.   

Abstract

HPLC analysis of sialic acids released from recombinant variants of human tissue plasminogen activator, human chimeric plasminogen activator, human erythropoietin, and human follitropin, expressed in Chinese hamster ovary cells, demonstrates for each glycoprotein the presence of N-acetylneuraminic acid and N-glycolylneuraminic acid in a ratio of 97:3. Structural analysis by 500 MHz1H-NMR spectroscopy, of the enzymatically released N-linked carbohydrate chains of chimeric plasminogen activator and of erythropoietin, showed that alpha 2-3 linked N-glycolylneuraminic acid can occur in different N-acetyllactosamine type antennary structures.

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Year:  1990        PMID： 2124546     DOI： 10.1016/0014-5793(90)81427-p

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  16 in total

1.  Probing the frontiers of glycoprotein synthesis: the fully elaborated β-subunit of the human follicle-stimulating hormone.

Authors:  Pavel Nagorny; Neeraj Sane; Bernhard Fasching; Baptiste Aussedat; Samuel J Danishefsky
Journal:  Angew Chem Int Ed Engl       Date:  2011-12-09       Impact factor: 15.336

2.  The role of CMP-N-acetylneuraminic acid hydroxylase in determining the level of N-glycolylneuraminic acid in porcine tissues.

Authors:  Y N Malykh; L Shaw; R Schauer
Journal:  Glycoconj J       Date:  1998-09       Impact factor: 2.916

3.  Implications of the presence of N-glycolylneuraminic acid in recombinant therapeutic glycoproteins.

Authors:  Darius Ghaderi; Rachel E Taylor; Vered Padler-Karavani; Sandra Diaz; Ajit Varki
Journal:  Nat Biotechnol       Date:  2010-07-25       Impact factor: 54.908

Review 4.  Analytical tools for characterizing biopharmaceuticals and the implications for biosimilars.

Authors:  Steven A Berkowitz; John R Engen; Jeffrey R Mazzeo; Graham B Jones
Journal:  Nat Rev Drug Discov       Date:  2012-06-29       Impact factor: 84.694

5.  Comparison of the carbohydrate moieties of recombinant soluble Fc epsilon receptor (sFc epsilon RII/sCD23) expressed in Saccharomyces cerevisiae and Chinese hamster ovary cells. Different O-glycosylation sites are used by yeast and mammalian cells.

Authors:  I Kalsner; F J Schneider; R Geyer; H Ahorn; I Maurer-Fogy
Journal:  Glycoconj J       Date:  1992-08       Impact factor: 2.916

Review 6.  Sialic acids in human health and disease.

Authors:  Ajit Varki
Journal:  Trends Mol Med       Date:  2008-07-06       Impact factor: 11.951

7.  Toward fully synthetic homogeneous beta-human follicle-stimulating hormone (beta-hFSH) with a biantennary N-linked dodecasaccharide. synthesis of beta-hFSH with chitobiose units at the natural linkage sites.

Authors:  Pavel Nagorny; Bernhard Fasching; Xuechen Li; Gong Chen; Baptiste Aussedat; Samuel J Danishefsky
Journal:  J Am Chem Soc       Date:  2009-04-29       Impact factor: 15.419

Review 8.  Human risk of diseases associated with red meat intake: Analysis of current theories and proposed role for metabolic incorporation of a non-human sialic acid.

Authors:  Frederico Alisson-Silva; Kunio Kawanishi; Ajit Varki
Journal:  Mol Aspects Med       Date:  2016-07-12

9.  LC-MS/MS peptide mapping with automated data processing for routine profiling of N-glycans in immunoglobulins.

Authors:  Bhavana Shah; Xinzhao Grace Jiang; Louise Chen; Zhongqi Zhang
Journal:  J Am Soc Mass Spectrom       Date:  2014-03-25       Impact factor: 3.109

10.  A new recombinant factor VIII: from genetics to clinical use.

Authors:  Christoph Kannicht; Guido Kohla; Maya Tiemeyer; Olaf Walter; Helena Sandberg
Journal:  Drug Des Devel Ther       Date:  2015-07-23       Impact factor: 4.162
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