AIM: This in vitro study was established to investigate whether the regenerative capacity of periodontal ligament (PDL) cells in the presence of enamel matrix derivative (EMD) is modulated by inflammation. MATERIALS AND METHODS: PDL cells were grown in the presence or absence of EMD under normal and inflammatory conditions for up to 14 days. In order to mimic an inflammatory environment, cells were incubated with interleukin (IL)-1β. Cells were also exposed to transforming growth factor (TGF)-β1 and insulin-like growth factor (IGF)-1 under both conditions. For analysis of wound healing, an in vitro wound fill assay was used. The synthesis of growth factors, markers of proliferation, and osteogenic differentiation, as well as collagen was studied by real-time polymerase chain reaction, enzyme-linked immunoassay, and immunoblotting. Mineralization was assessed by alizarine red S and von Kossa staining. RESULTS: EMD stimulated significantly the in vitro wound fill rate, cell proliferation and adhesion, synthesis of growth factors, and collagen, as well as mineralization. In the presence of IL-1β, these EMD effects were significantly reduced. IL-1β also inhibited significantly the wound fill rate induced by TGF-β1 and IGF-1. CONCLUSIONS: Critical PDL cell functions that are associated with periodontal regeneration are reduced in an inflammatory environment.
AIM: This in vitro study was established to investigate whether the regenerative capacity of periodontal ligament (PDL) cells in the presence of enamel matrix derivative (EMD) is modulated by inflammation. MATERIALS AND METHODS: PDL cells were grown in the presence or absence of EMD under normal and inflammatory conditions for up to 14 days. In order to mimic an inflammatory environment, cells were incubated with interleukin (IL)-1β. Cells were also exposed to transforming growth factor (TGF)-β1 and insulin-like growth factor (IGF)-1 under both conditions. For analysis of wound healing, an in vitro wound fill assay was used. The synthesis of growth factors, markers of proliferation, and osteogenic differentiation, as well as collagen was studied by real-time polymerase chain reaction, enzyme-linked immunoassay, and immunoblotting. Mineralization was assessed by alizarine red S and von Kossa staining. RESULTS: EMD stimulated significantly the in vitro wound fill rate, cell proliferation and adhesion, synthesis of growth factors, and collagen, as well as mineralization. In the presence of IL-1β, these EMD effects were significantly reduced. IL-1β also inhibited significantly the wound fill rate induced by TGF-β1 and IGF-1. CONCLUSIONS: Critical PDL cell functions that are associated with periodontal regeneration are reduced in an inflammatory environment.
Authors: Sarah Grimm; Eva Wolff; Christian Walter; Andreas M Pabst; Ambili Mundethu; Cornelius Jacobs; Heiner Wehrbein; Collin Jacobs Journal: Clin Oral Investig Date: 2019-05-17 Impact factor: 3.573
Authors: Svenja Memmert; A V B Nogueira; A Damanaki; M Nokhbehsaim; S Eick; T Divnic-Resnik; A Spahr; B Rath-Deschner; A Till; W Götz; J A Cirelli; A Jäger; J Deschner Journal: Clin Oral Investig Date: 2018-02-13 Impact factor: 3.573
Authors: Richard J Miron; Oana M Caluseru; Vincent Guillemette; Yufeng Zhang; Anja C Gemperli; Fatiha Chandad; Anton Sculean Journal: PLoS One Date: 2013-08-12 Impact factor: 3.240
Authors: Marjan Nokhbehsaim; Sigrun Eick; Andressa Vilas Boas Nogueira; Per Hoffmann; Stefan Herms; Holger Fröhlich; Søren Jepsen; Andreas Jäger; Joni Augusto Cirelli; James Deschner Journal: Mediators Inflamm Date: 2013-08-22 Impact factor: 4.711
Authors: Marjan Nokhbehsaim; Sema Keser; Andressa Vilas Boas Nogueira; Joni Augusto Cirelli; Søren Jepsen; Andreas Jäger; Sigrun Eick; James Deschner Journal: J Diabetes Res Date: 2014-07-13 Impact factor: 4.011
Authors: Marjan Nokhbehsaim; Sema Keser; Andressa Vilas Boas Nogueira; Andreas Jäger; Søren Jepsen; Joni Augusto Cirelli; Christoph Bourauel; Sigrun Eick; James Deschner Journal: Int J Endocrinol Date: 2014-07-22 Impact factor: 3.257
Authors: Anna Damanaki; Marjan Nokhbehsaim; Sigrun Eick; Werner Götz; Jochen Winter; Gerhard Wahl; Andreas Jäger; Søren Jepsen; James Deschner Journal: Mediators Inflamm Date: 2014-02-25 Impact factor: 4.711