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Literature DB >> 2123547

Purification and immunologic reactivity of Hymenolepis diminuta surface antigens.

Abstract

The outer part of Hymenolepis diminuta tegument was extracted with 3 M KCl. The antigen was adsorbed from the extract on an affinity column containing H. diminuta-infected rat immunoglobulin immobilized on Sepharose 4B and could be eluted with 2.5 M urea at pH 2.8. Analysis of polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of both the crude extract and material eluted from the column showed that the latter was markedly purified. Double diffusion and immunoelectrophoresis revealed 11 and 4 antigenic components in the crude extract and purified preparation, respectively.

Entities: Chemical Species

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Year: 1990 PMID： 2123547 DOI： 10.1007/bf00931091

Source DB: PubMed Journal: Parasitol Res ISSN： 0932-0113 Impact factor: 2.289

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References

16 in total

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Authors: D D Isaak
Journal: J Helminthol Date: 1983-03 Impact factor: 2.170

Purification and immunologic reactivity of Hymenolepis diminuta surface antigens.

1. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

2. Isolation and partial biochemical characterization of the brush border plasma membrane from the cestode, Hymenolepis diminuta.

3. Influence of 0.02 M EDTA and 3 M KCl on surface of Hymenolepis diminuta and composition of isolated proteins.

4. Hymenolepis diminuta and H. microstoma: mouse immunoglobulins binding to the tegumental surface.

5. The immunoglobulin coat on Hymenolepis spp.

6. Antibody response to tapeworm (Hymenolepis diminuta) in the rat.

7. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

8. Immunoregulatory factors derived from human tumors. II. Partial purification and further immunobiochemical characterization of a human sarcoma-derived immunosuppressive factor expressing HLA-DR and immunoglobulin-related determinants.

Review 9. The antigenic fractions of Hymenolepis diminuta; their usefulness in immunological examinations.

10. In vitro tapeworm extract-induced proliferative responses of gut-associated lymphoid cells from Hymenolepis diminuta infected mice.